PMID: 11927382Apr 3, 2002Paper

Overexpression of vasopressin in the rat transgenic for the metallothionein-vasopressin fusion gene

The Journal of Endocrinology
H NagasakiY Oiso

Abstract

Arginine vasopressin (AVP) is a major antidiuretic hormone, the overproduction of which causes diluting hyponatremia in humans and is called the syndrome of inappropriate antidiuresis (SIAD). To study physiological changes resulting from AVP overproduction and to develop an animal model of hyponatremia, the human AVP gene was expressed under the control of the metallothionein promoter in transgenic (Tg) rats. Analyses of AVP immunoreactivity (irAVP) in the tissues revealed that the transgene is expressed mainly in the central nervous system. Gel filtration showed that irAVP in the brain and plasma was properly processed AVP. AVP purified from the brains of both Tg and control rats also exerted equal bioactivity to generate cAMP in LLC-PK1 cells. The founder rats did not show any physical or anatomical abnormalities. Under basal conditions, Tg rats had high plasma AVP levels (Tg 13.8 +/- 2.5 pg/ml; control 2.7 +/- 1.2 pg/ml; n=6 in both groups; means +/- S.E.M.), decreased urine volume, and normal plasma [Na(+)]. Hypertonic saline injected i.p. did not affect AVP secretion in Tg rats. In response to a zinc-supplemented liquid diet, plasma AVP decreased in control rats, but increased in Tg rats (Tg 32.7 +/- 2.7 pg/ml; control 1.0...Continue Reading

Citations

Oct 26, 2005·Transgenic Research·Laurent TessonIgnacio Anegon
Feb 26, 2003·European Journal of Pharmacology·Gonzalo A Carrasco, Louis D Van de Kar
Jun 5, 2003·American Journal of Physiology. Cell Physiology·Atsuko MizunoMakoto Suzuki
Aug 14, 2003·American Journal of Physiology. Endocrinology and Metabolism·Kazushige TachikawaYutaka Oiso

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