Overexpression of wound-responsive RNA-binding proteins induces leaf senescence and hypersensitive-like cell death.

The New Phytologist
Cha Young KimSarah M Assmann

Abstract

Leaf senescence is a form of programmed cell death, and involves regulated expression of a specific set of senescence-associated genes (SAGs). In Arabidopsis, three UBA2 genes, UBA2a, UBA2b, and UBA2c, encode heterogeneous nuclear ribonucleoprotein (hnRNP)-type RNA-binding proteins. Previously, it has been demonstrated that expression of UBA2 genes is induced by mechanical wounding in a splice variant-dependent manner. Constitutive overexpression of the UBA2 genes proved lethal. Accordingly, a conditional gain-of-function system was used here to assess phenotypes related to UBA2 overexpression. Overexpression of each of the three UBA2 genes leads to a leaf yellowing/cell death-like phenotype in Arabidopsis plants. Expression levels of a number of SAGs, such as SAG13, SAG14, SAG15, SAG101, WRKY6, WRKY53, WRKY70, ACS2, ACS6, CML38 and SIRK, were elevated upon induction of UBA2 overexpression, as were transcripts of multiple wounding- and defense-related genes, including EDS1, CK1, JR1, WR3 and MPK3. Elevated ethylene biosynthesis and hypersensitive-like patterns of cell death and callose deposition, shown by Trypan blue and aniline blue staining, respectively, were also observed following induced overexpression of UBA2a, UBA2b, a...Continue Reading

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Citations

Oct 4, 2011·Journal of Molecular Evolution·Toni I Gossmann, Karl J Schmid
Nov 8, 2012·Journal of Integrative Plant Biology·Xiao-Yuan WuHai-Chun Jing
Sep 17, 2013·The New Phytologist·Biswa R AcharyaSarah M Assmann
Mar 19, 2013·Plant Science : an International Journal of Experimental Plant Biology·Ian M SilvermanBrian D Gregory
Sep 16, 2014·Plant Science : an International Journal of Experimental Plant Biology·Wan-Chin YeapChai-Ling Ho
Jul 14, 2016·Scientific Reports·Claudius MarondedzeChris Gehring
Oct 14, 2017·Nature Communications·Maciej MaselkoMichael J Smanski
May 27, 2020·Nature Ecology & Evolution·Laura J KellyRichard J A Buggs
Nov 26, 2020·Journal of Proteomics·Kishor D IngoleSaikat Bhattacharjee

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