Overexpression of ZmIRT1 and ZmZIP3 Enhances Iron and Zinc Accumulation in Transgenic Arabidopsis

PloS One
Suzhen LiRumei Chen

Abstract

Iron and zinc are important micronutrients for both the growth and nutrient availability of crop plants, and their absorption is tightly controlled by a metal uptake system. Zinc-regulated transporters, iron-regulated transporter-like proteins (ZIP), is considered an essential metal transporter for the acquisition of Fe and Zn in graminaceous plants. Several ZIPs have been identified in maize, although their physiological function remains unclear. In this report, ZmIRT1 was shown to be specifically expressed in silk and embryo, whereas ZmZIP3 was a leaf-specific gene. Both ZmIRT1 and ZmZIP3 were shown to be localized to the plasma membrane and endoplasmic reticulum. In addition, transgenic Arabidopsis plants overexpressing ZmIRT1 or ZmZIP3 were generated, and the metal contents in various tissues of transgenic and wild-type plants were examined based on ICP-OES and Zinpyr-1 staining. The Fe and Zn concentration increased in roots and seeds of ZmIRT1-overexpressing plants, while the Fe content in shoots decreased. Overexpressing ZmZIP3 enhanced Zn accumulation in the roots of transgenic plants, while that in shoots was repressed. In addition, the transgenic plants showed altered tolerance to various Fe and Zn conditions compared...Continue Reading

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Citations

Mar 29, 2016·Plant Signaling & Behavior·Suzhen LiRumei Chen
Mar 2, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Alexey P OrlovDmitry A Kuznetsov
Feb 9, 2019·Journal, Genetic Engineering & Biotechnology·Saikat Gantait, Suvendu Mondal
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Oct 31, 2017·Frontiers in Plant Science·Grace Z H TanSagadevan G Mundree
Mar 31, 2018·Frontiers in Plant Science·Xiaofen YuGuangyuan He
Jun 17, 2020·Frontiers in Plant Science·T P Ajeesh KrishnaStanislaus Antony Ceasar
Jun 11, 2017·Journal of Integrative Plant Biology·Liyu Chen, Hong Liao

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Methods Mentioned

BETA
transgenic
PCR

Software Mentioned

ZEN
ABI7500
ImageJ

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