Overexpression, purification and crystallization of bacteriocin LlpA from Pseudomonas sp. BW11M1

Acta Crystallographica. Section D, Biological Crystallography
Annabel H A ParretRemy Loris

Abstract

LlpA is a bacteriocin produced by Pseudomonas sp. BW11M1 that shows remarkable similarity to a family of mannose-binding plant lectins. A His-tagged version of LlpA was recombinantly produced in Escherichia coli and purified to homogeneity. Single crystals were grown by vapour diffusion and belong to space group P2(1)2(1)2, with unit-cell parameters a = 150.5, b = 154.5, c = 34.2 A. The crystals diffract to at least 2.2 A using synchrotron radiation.

Citations

Sep 10, 2005·Applied and Environmental Microbiology·Annabel H A ParretRené De Mot
Mar 8, 2013·PLoS Pathogens·Maarten G K GhequireRené De Mot
Jun 14, 2013·Environmental Microbiology Reports·Maarten G K GhequireRené De Mot
Jun 6, 2013·MicrobiologyOpen·Maarten G K GhequireRené De Mot
Sep 17, 2014·MicrobiologyOpen·Maarten G K GhequireRené De Mot
Jun 14, 2014·FEMS Microbiology Reviews·Maarten G K Ghequire, René De Mot
Nov 28, 2012·Biochemical Society Transactions·Rhys GrinterDaniel Walker
Jun 16, 2018·Microbiology·Fernando Uriel Rojas-RojasPaulina Estrada-de Los Santos
Jan 15, 2021·Comprehensive Reviews in Food Science and Food Safety·Yanlong CuiXin Lü

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