Overexpression, purification, crystallization and preliminary diffraction studies of the Protaminobacter rubrum sucrose isomerase SmuA

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Stéphanie RavaudNushin Aghajari

Abstract

Palatinose (isomaltulose, alpha-D-glucosylpyranosyl-1,6-D-fructofuranose), a nutritional and acariogenic reducing sugar, is industrially obtained from sucrose by using immobilized cells of Protaminobacter rubrum that produce the sucrose isomerase SmuA. The isomerization of sucrose catalyzed by this enzyme also results in the formation of trehalulose (alpha-D-glucosylpyranosyl-1,1-D-fructofuranose) in smaller amounts and glucose, fructose and eventually isomaltose as by-products, which lower the yield of the reaction and complicate the recovery of palatinose. The determination of the three-dimensional structure of SmuA will provide a basis for rational protein-engineering studies in order to optimize the industrial production of palatinose. A recombinant form of the 67.3 kDa SmuA enzyme has been crystallized in the native state by the vapour-diffusion method. Crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 61.6, b = 81.4, c = 135.6 A, and diffract to 1.95 A resolution on a synchrotron-radiation source.

References

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Oct 1, 1994·Bioscience, Biotechnology, and Biochemistry·Y NagaiK Tsuyuki
Sep 22, 2001·Acta Crystallographica. Section D, Biological Crystallography·J Navaza
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Jun 24, 2003·The Journal of Biological Chemistry·Daohai ZhangKunchithapadam Swaminathan
Mar 2, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Stéphanie RavaudNushin Aghajari

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Citations

May 29, 2014·Applied Microbiology and Biotechnology·Wanmeng MuBo Jiang
May 12, 2009·FEBS Letters·Stéphanie RavaudNushin Aghajari
Dec 3, 2011·Enzyme and Microbial Technology·Ken C GoulterRobert G Birch
Nov 30, 2011·Chembiochem : a European Journal of Chemical Biology·Julian GörlJürgen Seibel
Oct 7, 2019·Applied Microbiology and Biotechnology·Yuqing TianWanmeng Mu
Mar 18, 2020·Chembiochem : a European Journal of Chemical Biology·Patrick PilakArne Skerra

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