Overfeeding reduces insulin sensitivity and increases oxidative stress, without altering markers of mitochondrial content and function in humans.

PloS One
Dorit Samocha-BonetLeonie K Heilbronn

Abstract

Mitochondrial dysfunction and increased oxidative stress are associated with obesity and type 2 diabetes. High fat feeding induces insulin resistance and increases skeletal muscle oxidative stress in rodents, but there is controversy as to whether skeletal muscle mitochondrial biogenesis and function is altered. Forty (37 ± 2 y) non-obese (25.6 ± 0.6 kg/m(2)) sedentary men (n = 20) and women (n = 20) were overfed (+1040 ± 100 kcal/day, 46 ± 1% of energy from fat) for 28 days. Hyperinsulinemic-euglycemic clamps were performed at baseline and day 28 of overfeeding and skeletal muscle biopsies taken at baseline, day 3 and day 28 of overfeeding in a sub cohort of 26 individuals (13 men and 13 women) that consented to having all 3 biopsies performed. Weight increased on average in the whole cohort by 0.6 ± 0.1 and 2.7 ± 0.3 kg at days 3 and 28, respectively (P<0.0001, without a significant difference in the response between men and women (P = 0.4). Glucose infusion rate during the hyperinsulinemic-euglycemic clamp decreased from 54.8 ± 2.8 at baseline to 50.3 ± 2.5 µmol/min/kg FFM at day 28 of overfeeding (P = 0.03) without a significant difference between men and women (P = 0.4). Skeletal muscle protein carbonyls and urinary F2-iso...Continue Reading

Associated Clinical Trials

Nov 22, 2007·Dr Jerry Greenfield
Jul 17, 2015·Dorit Samocha-Bonet

References

Jan 1, 1984·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·W C MillerR K Conlee
Jan 16, 2002·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·S IossaG Liverini
Dec 6, 2002·Pflügers Archiv : European journal of physiology·Susanna IossaGiovanna Liverini
Dec 4, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Paolo MontuschiL Jackson Roberts
Jan 25, 2007·The Journal of Clinical Endocrinology and Metabolism·Leonie K HeilbronnDonald J Chisholm
Apr 23, 2008·European Journal of Endocrinology·H M De FeyterJ J Prompers
May 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Chad R HancockJohn O Holloszy
Sep 23, 2008·Trends in Endocrinology and Metabolism : TEM·Nigel Turner, Leonie K Heilbronn
Feb 4, 2009·The Journal of Clinical Investigation·Ethan J AndersonP Darrell Neufer
Apr 1, 2009·The Journal of Physiology·Charlotte BrønsAllan Vaag
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kyle L HoehnDavid E James
Oct 8, 2009·Cell Metabolism·Kim LohTony Tiganis
Oct 27, 2009·Trends in Endocrinology and Metabolism : TEM·Dorit Samocha-BonetLesley V Campbell
Feb 11, 2010·Journal of Proteome Research·Sander L J WijersWouter D van Marken Lichtenbelt
May 6, 2010·American Journal of Physiology. Endocrinology and Metabolism·A BrehmM Roden
Apr 5, 2012·Journal of Nutrition and Metabolism·S GregersenL V Campbell

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Citations

Mar 16, 2013·Advances in Nutrition·Brigitte I Frohnert, David A Bernlohr
Aug 25, 2015·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Rebecca S WilliamsDorit Samocha-Bonet
Jan 26, 2016·Nutrition & Diabetes·A K ElshorbagyL K Heilbronn
May 4, 2013·Obesity·Leonie K HeilbronnDorit Samocha-Bonet
Jul 26, 2014·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·D Samocha-BonetE Ravussin
Oct 25, 2014·Journal of Pharmacological Sciences·Tae-Jin JuSo-Young Park
Dec 21, 2013·Diabetes Care·Dorit Samocha-BonetLeonie Kaye Heilbronn
Jun 16, 2016·Antioxidants & Redox Signaling·Schohraya SpahisEmile Levy
Nov 19, 2017·Diabetologia·Frederico G S ToledoEric Ravussin
Aug 7, 2018·Journal of Developmental Origins of Health and Disease·C JuvetH Chehade
Apr 2, 2014·The Journal of Clinical Endocrinology and Metabolism·K SeysselH Vidal
Jul 29, 2018·Journal of Lipid Research·Daniel J FazakerleyDavid E James
Feb 1, 2020·Journal of Cachexia, Sarcopenia and Muscle·Yasuhiro NishidaKazuyuki Tobe
May 10, 2016·The Journal of Clinical Endocrinology and Metabolism·George A BraySteven R Smith
Feb 25, 2017·The Journal of Endocrinology·Sergio Di MeoPaola Venditti
Sep 3, 2020·European Journal of Clinical Nutrition·Eliza Chalmers, Dorit Samocha-Bonet
Jun 10, 2020·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·George A Bray, Claude Bouchard
Jul 12, 2020·European Journal of Clinical Nutrition·Erin I WalshNicolas Cherbuin
Feb 9, 2013·The Journal of Clinical Endocrinology and Metabolism·Jose E GalganiCedric Moro
Oct 11, 2020·Nature Reviews. Cardiology·Pedro L ValenzuelaAlejandro Lucia
Oct 9, 2020·The Journal of Clinical Endocrinology and Metabolism·Bo LiuLeonie K Heilbronn
Sep 19, 2020·Endocrinology·Manasi DasNicholas J G Webster
Oct 9, 2020·Yeungnam University Journal of Medicine·Soyoung Park, So-Young Park
Mar 21, 2019·Journal of Proteome Research·Dylan J HarneyMark Larance

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Methods Mentioned

BETA
biopsy

Software Mentioned

SPSS Statistics

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