Overhydroxylation of Lysine of Collagen Increases Uterine Fibroids Proliferation: Roles of Lysyl Hydroxylases, Lysyl Oxidases, and Matrix Metalloproteinases

BioMed Research International
Marwa KamelSalama A Salama

Abstract

The role of the extracellular matrix (ECM) in uterine fibroids (UF) has recently been appreciated. Overhydroxylation of lysine residues and the subsequent formation of hydroxylysylpyridinoline (HP) and lysylpyridinoline (LP) cross-links underlie the ECM stiffness and profoundly affect tumor progression. The aim of the current study was to investigate the relationship between ECM of UF, collagen and collagen cross-linking enzymes [lysyl hydroxylases (LH) and lysyl oxidases (LOX)], and the development and progression of UF. Our results indicated that hydroxyl lysine (Hyl) and HP cross-links are significantly higher in UF compared to the normal myometrial tissues accompanied by increased expression of LH (LH2b) and LOX. Also, increased resistance to matrix metalloproteinases (MMP) proteolytic degradation activity was observed. Furthermore, the extent of collagen cross-links was positively correlated with the expression of myofibroblast marker (α-SMA), growth-promoting markers (PCNA; pERK1/2; FAKpY397; Ki-67; and Cyclin D1), and the size of UF. In conclusion, our study defines the role of overhydroxylation of collagen and collagen cross-linking enzymes in modulating UF cell proliferation, differentiation, and resistance to MMP. The...Continue Reading

References

Oct 20, 1975·Biochimica Et Biophysica Acta·A J BaileyA Delaunay
Apr 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S Ricard-BlumJ A Grimaud
Aug 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A López-De León, M Rojkind
Jul 1, 1989·The American Review of Respiratory Disease·G RaghuA S Narayanan
Nov 1, 1981·European Journal of Biochemistry·R TimplK Kühn
Feb 1, 1993·The Journal of Cell Biology·R L Juliano, S Haskill
Apr 1, 1997·BioTechniques·L B CreemersV Everts
Mar 21, 1998·Matrix Biology : Journal of the International Society for Matrix Biology·R A BankJ M te Koppele
Jan 12, 1999·Mechanisms of Ageing and Development·A J BaileyL Knott
Jun 7, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M I UzelP C Trackman
Dec 2, 2000·Molecular Human Reproduction·M UzumcuD R Brigstock
Mar 9, 2004·The Journal of Biological Chemistry·Seiji OhashiToshio Doi
Jun 11, 2005·Science·Cheryl Lyn Walker, Elizabeth A Stewart
Jun 16, 2005·Biochimica Et Biophysica Acta·Annemarie J van der SlotAnne-Marie Zuurmond
Feb 10, 2006·Journal of Cell Science·Heli RuotsalainenRaili Myllylä
Jul 25, 2006·Nature Reviews. Cancer·Andrew I Minchinton, Ian F Tannock
Aug 16, 2006·Cellular and Molecular Life Sciences : CMLS·H A Lucero, H M Kagan
Feb 10, 2007·Matrix Biology : Journal of the International Society for Matrix Biology·Kati TakaluomaJohanna Myllyharju
May 2, 2007·Journal of Cellular Biochemistry·Stacey L PayneDawn A Kirschmann
Oct 19, 2007·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Michał BogusiewiczTomasz Rechberger
Oct 25, 2007·Biochemical Society Transactions·S P Robins
Mar 1, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Rebecca G Wells
Apr 9, 2008·American Journal of Obstetrics and Gynecology·Rebecca RogersJames H Segars
Apr 30, 2008·Methods : a Companion to Methods in Enzymology·David R EyreJiann-Jiu Wu
Sep 22, 2009·Current Biology : CB·Eric A KleinRichard K Assoian
Dec 8, 2009·Science·Richard O Hynes
Feb 26, 2010·The Journal of Biological Chemistry·Takumi MaruhashiAkira Kudo
Apr 24, 2010·Seminars in Reproductive Medicine·Minnie MalikWilliam H Catherino
May 18, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Begoña LópezJavier Díez
Jul 27, 2011·Human Reproduction Update·Pasquapina CiarmelaMario Castellucci
Oct 11, 2011·Matrix Biology : Journal of the International Society for Matrix Biology·John M NorianJames H Segars
Jan 17, 2012·American Journal of Obstetrics and Gynecology·Eden R CardozoJames H Segars

❮ Previous
Next ❯

Citations

Feb 7, 2018·The Journal of Clinical Endocrinology and Metabolism·Guillaume E CourtoyMarie-Madeleine Dolmans
Apr 10, 2019·Anti-cancer Agents in Medicinal Chemistry·Shadab AnjumSaima Wajid
Dec 12, 2018·Journal of Cellular and Molecular Medicine·Heidi SchilterWolfgang Jarolimek
Dec 7, 2018·International Journal of Molecular Sciences·Michał CiebieraGrzegorz Jakiel

❮ Previous
Next ❯

Methods Mentioned

BETA
hysterectomy
PCR
electrophoresis
hysterectomies

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.