Overlapping repressor binding sites result in additive regulation of Escherichia coli FadH by FadR and ArcA.

Journal of Bacteriology
Youjun Feng, J E Cronan

Abstract

Escherichia coli fadH encodes a 2,4-dienoyl reductase that plays an auxiliary role in beta-oxidation of certain unsaturated fatty acids. In the 2 decades since its discovery, FadH biochemistry has been studied extensively. However, the genetic regulation of FadH has been explored only partially. Here we report mapping of the fadH promoter and document its complex regulation by three independent regulators, the fatty acid degradation FadR repressor, the oxygen-responsive ArcA-ArcB two-component system, and the cyclic AMP receptor protein-cyclic AMP (CRP-cAMP) complex. Electrophoretic mobility shift assays demonstrated that FadR binds to the fadH promoter region and that this binding can be specifically reversed by long-chain acyl-coenzyme A (CoA) thioesters. In vivo data combining transcriptional lacZ fusion and real-time quantitative PCR (qPCR) analyses indicated that fadH is strongly repressed by FadR, in agreement with induction of fadH by long-chain fatty acids. Inactivation of arcA increased fadH transcription by >3-fold under anaerobic conditions. Moreover, fadH expression was increased 8- to 10-fold under anaerobic conditions upon deletion of both the fadR and the arcA gene, indicating that anaerobic expression is additiv...Continue Reading

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Aug 18, 2009·Journal of Bacteriology·Youjun Feng, John E Cronan

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Citations

Jun 5, 2013·Journal of Bacteriology·Youjun FengSwaminath Srinivas
Oct 4, 2013·Journal of Molecular Microbiology and Biotechnology·Chun-Yi LinMasayori Inouye
Jan 23, 2015·MicrobiologyOpen·Huimin ZhangYoujun Feng
Dec 10, 2013·Molecular Microbiology·Youjun FengJohn E Cronan
May 13, 2014·Research in Microbiology·Youjun Feng, John E Cronan
Oct 8, 2014·Molecular Microbiology·Qing TangYoujun Feng
Mar 19, 2013·Progress in Lipid Research·Joshua B Parsons, Charles O Rock
Mar 27, 2018·Microbiology and Immunology·Yuto KawaiSatoshi Tsuneda
Nov 2, 2014·Molecular BioSystems·Joris BeldMichael D Burkart
Jan 10, 2018·Frontiers in Cellular and Infection Microbiology·Rongsui GaoYoujun Feng
May 11, 2017·MBio·Jian SunYoujun Feng
Feb 14, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Qingjing WangYoujun Feng
Apr 20, 2019·Applied Microbiology and Biotechnology·Jun YangXiaoyuan Wang
May 13, 2018·Applied and Environmental Microbiology·Julie L StoudenmireEric V Stabb

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