PMID: 2116701Jul 1, 1990Paper

Overlay technique for direct detection and identification of haemolytic Listeria on selective plating medium. Comparison of five media

Zeitschrift Für Lebensmittel-Untersuchung Und -Forschung
L DominguezG Suarez

Abstract

An overlay technique is proposed for the identification and counting of haemolytic Listeria colonies directly on selective plating media. The technique was applied to different Listeria-selective plating media. In pure culture studies with collection strains, the overlay technique was more efficient and reliable for detection haemolytic Listeria species compared with the incorporation of blood into the agar. The efficacy of the overlay technique for the direct detection of haemolytic colonies of Listeria from raw milk samples was related to agar selectivity. The best results were obtained with Listeria-selective agar medium modified (LSAMM). Catalase assay, together with reactions for aesculin and tellurite, were useful and reliable criteria for the identification of Listeria. All colonies on LSAMM which were positive for catalase, tellurite and aesculin while those displaying typical haemolysis corresponded in most cases to L. monocytogenes.

References

Nov 1, 1986·Applied and Environmental Microbiology·W H Lee, D McClain
Nov 1, 1988·Applied and Environmental Microbiology·A E YousefE H Marth
Dec 1, 1988·Applied and Environmental Microbiology·M J LoessnerL A Shelef
May 1, 1984·Applied and Environmental Microbiology·L Dominguez RodriguezE Rodriguez Ferri
Oct 1, 1988·Journal of Food Protection·Judy A Fraser, William H Sperber
May 1, 1987·Journal of Food Protection·David W Hird
Mar 1, 1989·Journal of Food Protection·Pamela K Cassiday, Robert E Brackett

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Citations

Sep 1, 1992·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·J F Fernandez-GarayzabalL Dominguez
Jan 1, 1992·Applied and Environmental Microbiology·J F Fernandez-GarayzabalL Dominguez
Dec 1, 1994·Applied and Environmental Microbiology·R C McKellar

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