Aug 26, 1998

Overproduction of acetyl-coenzyme A synthetase isoenzymes in respiring Saccharomyces cerevisiae cells does not reduce acetate production after exposure to glucose excess

FEMS Microbiology Letters
P de Jong-GubbelsJ T Pronk

Abstract

To investigate whether the production of acetate which occurs after exposure of respiring Saccharomyces cerevisiae cells to excess glucose can be reduced by overproduction of acetyl-CoA synthetase (ACS, EC 6.2.1.1), the ACS1 and ACS2 genes were introduced on multi-copy plasmids. For each isoenzyme, the level in glucose-limited chemostat cultures was increased by 3-6-fold, relative to an isogenic reference strain. However, ACS overproduction did not result in a reduced production of acetate after a glucose pulse (100 mmol l-1) to these cultures. This indicates that a limited capacity of ACS is not the sole cause of acetate accumulation in S. cerevisiae.

Mentioned in this Paper

Ethanol
ACSL1 wt Allele
Saccharomyces cerevisiae allergenic extract
Glucose, (beta-D)-Isomer
ACSL1 Gene
Acetyl-coenzyme A Synthetase
ACSL6 gene
Genes, Fungal
Yeast Proteins
Plasmids

About this Paper

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