Overproduction of nitrogenase by nitrogen-limited cultures of Rhodopseudomonas palustris.

Journal of Bacteriology
D J Arp, W G Zumft

Abstract

Rhodopseudomonas palustris cells grown on limiting nitrogen produced four- to eightfold higher nitrogenase specific activity relative to cells sparged with N2. The high activity of N-limited cells was the result of overproduction of the nitrogenase proteins. This was shown by four independent techniques: (i) titration of the Mo-Fe protein in cell-free extracts with Fe protein from Azotobacter vinelandii; (ii) direct detection of the subunits of Mo-Fe protein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis; (iii) monitoring of the electron paramagnetic resonance spectrum of Mo-Fe protein in whole cells; and (iv) immunological assay of the Fe protein level with an antiserum against the homologous protein of Rhodospirillum rubrum. The derepressed level of nitrogenase found in N2-grown cells was not due to an increased turnover of nitrogenase. The apparent half-lives of nitrogenase in N2-grown and N-limited cells were 58 and 98 h, respectively, but were too long to account for the difference in enzyme level. Half-lives were determined by measuring nitrogenase after repression of de novo synthesis by ammonia and subsequent release of nitrogenase switch-off by methionine sulfoximine. Observations were extended to R. rubr...Continue Reading

References

Nov 2, 1976·Archives of Microbiology·N M Weare, K T Shanmugam
Jan 1, 1978·Current Topics in Cellular Regulation·L E Mortenson
Feb 1, 1977·Journal of General Microbiology·J R Postgate, V Krishnapillai
Oct 1, 1975·Journal of Bacteriology·J E BrenchleyL G Patil
Jan 1, 1977·Applied and Environmental Microbiology·J C Weissman, J R Benemann
Apr 27, 1978·Archives of Microbiology·W G Zumft, F Castillo
Jun 1, 1969·Journal of General Microbiology·H Dalton, J R Postgate
Feb 1, 1981·Journal of Bacteriology·W J Sweet, R H Burris
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G B Ruvkun, F M Ausubel
Sep 1, 1980·Microbiological Reviews·W J Brill
Jan 1, 1981·Molecular & General Genetics : MGG·G R Wei, S Kustu

❮ Previous
Next ❯

Citations

Jan 1, 1987·Critical Reviews in Microbiology·P C Hallenbeck
Aug 1, 1985·Archives of Microbiology·J R Postgate, H M Kent
Nov 26, 1999·Journal of Bacteriology·B BrewinM Drummond
Jan 1, 1984·Journal of Bacteriology·M MadiganR A Stegeman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.