Overproduction of Sch9 leads to its aggregation and cell elongation in Saccharomyces cerevisiae

PloS One
Polina DrozdovaStanislav Bondarev

Abstract

The Sch9 kinase of Saccharomyces cerevisiae is one of the major TOR pathway effectors and regulates diverse processes in the cell. Sch9 belongs to the AGC kinase family. In human, amplification of AGC kinase genes is connected with cancer. However, not much is known about the effects of Sch9 overproduction in yeast cells. To fill this gap, we developed a model system to monitor subcellular location and aggregation state of overproduced Sch9 or its regions fused to a fluorescent protein. With this system, we showed that Sch9-YFP forms detergent-resistant aggregates, and multiple protein regions are responsible for this. This finding corroborated the fact that Sch9-YFP is visualized as various fluorescent foci. In addition, we found that Sch9 overproduction caused cell elongation, and this effect was determined by its C-terminal region containing kinase domains. The constructs we present can be exploited to create superior yeast-based model systems to study processes behind kinase overproduction in cancers.

References

Jan 2, 1992·Gene·T W ChristiansonP Hieter
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S Munro, T Maniatis
Jan 1, 1997·Trends in Biochemical Sciences·T Hunter, G D Plowman
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·M D Michelitsch, J S Weissman
May 21, 2003·Breast Cancer Research and Treatment·Colleen S SinclairFergus J Couch
Sep 26, 2003·The Journal of Biological Chemistry·Dmitry S KryndushkinVitaly V Kushnirov
Jul 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Toru NakayashikiReed B Wickner
Feb 14, 2006·Cell·Stephan WullschlegerMichael N Hall
Jun 16, 2006·Methods : a Companion to Methods in Enzymology·Vitaly V KushnirovMichael D Ter-Avanesyan
Jun 15, 2007·Molecular Cell·Jörg UrbanRobbie Loewith
Aug 19, 2007·Cancer Cell·Joan BruggeGordon B Mills
Aug 22, 2007·PloS One·Martin WeinbergerWilliam C Burhans
Aug 30, 2008·Nature·Daniel KaganovichJudith Frydman
Oct 19, 2010·Breast Cancer Research and Treatment·Gizeh Pérez-TenorioOlle Stål
Apr 5, 2011·Enzyme Research·Subbarao V Madhunapantula, Gavin P Robertson
Nov 8, 2011·Journal of Molecular Evolution·Teunis J P van DamBerend Snel
Feb 18, 2012·Nature·Randal HalfmannSusan Lindquist
Jun 7, 2012·G3 : Genes - Genomes - Genetics·Mark K Chee, Steven B Haase
Aug 11, 2012·Genetics·Susan W Liebman, Yury O Chernoff
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Dec 25, 2012·FEBS Letters·Abdullah B Ahmed, Andrey V Kajava
Jul 31, 2013·Frontiers in Oncology·Olivier E Pardo, Michael J Seckl
Aug 13, 2013·Frontiers in Oncology·Narayanasamy Arul, Yong-Yeon Cho
Nov 8, 2013·Molecular Biology of the Cell·Erwin SwinnenJoris Winderickx
Jan 21, 2014·Biochimica Et Biophysica Acta·Senena Corbalan-Garcia, Juan C Gómez-Fernández
Jan 25, 2014·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Antoine HollebecqueJean-Charles Soria
Aug 26, 2014·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·Abdullah B AhmedAndrey V Kajava
Oct 7, 2014·Cellular and Molecular Life Sciences : CMLS·Triana Amen, Daniel Kaganovich

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
restriction digest

Software Mentioned

IUPred
coin
ImageJ
ggplot2
R package
ArchCandy

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.