PMID: 9173878Apr 1, 1997Paper

Overt and latent activities of diacylglycerol acytransferase in rat liver microsomes: possible roles in very-low-density lipoprotein triacylglycerol secretion

The Biochemical Journal
M R OwenV A Zammit

Abstract

The possibility that triacylglycerol (TAG) synthesis occurs on both aspects of the endoplasmic-reticular membrane during the process of incorporation of TAG into secreted very-low-density lipoprotein (VLDL) [Zammit (1996) Biochem. J. 314, 1-14] was investigated by measuring the latency of diacylglycerol acyltransferase (DGAT) in microsomal fractions obtained from rat liver homogenates. Permeabilization of microsomes with taurocholate resulted in the doubling of the activity, indicating that DGAT activities of approximately equal magnitude occur on either aspect of the microsomal membrane. The taurocholate concentrations required for exposure of the latent activity of DGAT were identical with those that resulted in the exposure of marker enzymes for the lumen of the endoplasmic reticulum. Fractionation of the microsomes into smooth and rough populations indicated that the distribution of overt and latent DGAT activities was the same throughout. The possibility that taurocholate effects may result from non-specific activation of the overt enzyme was excluded by employing the channel-forming peptide alamethicin to effect permeabilization, and by varying the mode of delivery of diacylglycerol substrate to the microsomal membranes. ...Continue Reading

Citations

Jun 19, 2012·Progress in Lipid Research·Qin LiuRandall J Weselake
Jul 15, 2015·Cellular and Molecular Life Sciences : CMLS·Thomas Oliver Eichmann, Achim Lass
Oct 3, 2003·Biochemical and Biophysical Research Communications·Koji WakimotoYuji Imai
Aug 15, 2000·Annual Review of Nutrition·N O Davidson, G S Shelness
Aug 15, 2000·Annual Review of Nutrition·R A ColemanD M Muoio
May 25, 2013·Annual Review of Nutrition·Kelly V RugglesStephen L Sturley
Dec 16, 2006·Journal of Lipid Research·Jingsong CaoYuguang Shi
Sep 2, 2008·Journal of Lipid Research·Chi-Liang Eric YenRobert V Farese
Jun 16, 2012·Journal of Lipid Research·Stephen L Sturley, M Mahmood Hussain
Apr 19, 2008·Journal of Lipid Research·Judith StorchWilliam S Lagakos
Sep 18, 2002·Journal of Lipid Research·Ian J WatermanVictor A Zammit
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Jun 11, 2008·Cell Biochemistry and Function·Fábio Luís Tavares, Marília Cerqueira Leite Seelaender
Oct 14, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Julie TsaiKhosrow Adeli
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Sep 11, 1999·Current Opinion in Lipidology·S O OlofssonJ Borén

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