Overview of Fluorescence Lifetime Measurements in Flow Cytometry

Methods in Molecular Biology
Jessica P HoustonGiacomo Vacca

Abstract

The focus of this chapter is time-resolved flow cytometry, which is broadly defined as the ability to measure the timing of fluorescence decay from excited fluorophores that pass through cytometers or high-throughput cell counting and cell sorting instruments. We focus on this subject for two main reasons: first, to discuss the nuances of hardware and software modifications needed for these measurements because currently, there are no widespread time-resolved cytometers nor a one-size-fits-all approach; and second, to summarize the application space for fluorescence lifetime-based cell counting/sorting owing to the recent increase in the number of investigators interested in this approach. Overall, this chapter is structured into three sections: (1) theory of fluorescence decay kinetics, (2) modern time-resolved flow cytometry systems, and (3) cell counting and sorting applications. These commentaries are followed by conclusions and discussion about new directions and opportunities for fluorescence lifetime measurements in flow cytometry.

Citations

Aug 14, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Kapil NichaniJessica P Houston
Nov 12, 2020·Scientific Reports·Daniel KageUte Resch-Genger
Jun 16, 2021·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Jared HendersonRichard Eric Davis

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