Overview of peptide and protein analysis by mass spectrometry

Current Protocols in Molecular Biology
Steven A Carr, Roland S Annan


The goals of this unit are to familiarize peptide and protein chemists with the types of mass spectrometers that are appropriate for the majority of their analytical needs, to describe the kinds of experiments that can be performed with these instruments on a routine basis, and to discuss the kinds of information that these experiments provide. The emphasis here is on established tools and techniques that can realistically be used for problem solving. As a result, many useful instruments and techniques employed by the trained mass spectroscopist are not discussed here, because they do not satisfy these criteria or are not yet widely available. A few of these are briefly mentioned at the end of this introductory overview. The overview concludes with a tutorial discussion on the meaning and practical importance of a number of fundamental experimental and performance-related issues that have important implications for interpretation and use of mass spectral data, such as mass accuracy and resolution.


Dec 2, 1977·Science·R Uy, F Wold
Apr 1, 1992·Rapid Communications in Mass Spectrometry : RCM·Roland S AnnanK Biemann
Jan 1, 1992·Annual Review of Biochemistry·K Biemann
Dec 15, 1991·Analytical Chemistry·Steven A CarrG D Roberts
Jan 1, 1990·Methods in Enzymology·R A Yost, R K Boyd
Nov 1, 1988·Rapid Communications in Mass Spectrometry : RCM·T C CoveyJ Henion
Oct 1, 1988·Biomedical & Environmental Mass Spectrometry·K Biemann
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D F HuntC R Hauer
Jan 1, 1981·Annual Review of Biochemistry·F Wold
Jun 1, 1995·Trends in Biochemical Sciences·M Mann, M Wilm
Nov 1, 1994·Protein Science : a Publication of the Protein Society·R R LooP C Andrews
Apr 15, 1995·Analytical Chemistry·R S RushV Katta
Aug 1, 1993·Current Opinion in Biotechnology·R Aebersold
Feb 1, 1994·Current Opinion in Biotechnology·R Wang, B T Chait
Sep 1, 1994·Rapid Communications in Mass Spectrometry : RCM·M Bartlet-JonesD J Pappin
Feb 1, 1994·Protein Science : a Publication of the Protein Society·K PalczewskiK A Walsh
Jun 15, 1994·Analytical Chemistry·A L BurlingameS J Gaskell
Dec 1, 1993·Analytical Biochemistry·P JenöM Horst
Oct 1, 1993·Science·B T ChaitS B Kent
Nov 5, 1993·Science·S W Englander
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·W J HenzelC Watanabe

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Nodding Syndrome

Nodding Syndrome is a neurological and epileptiform disorder characterized by psychomotor, mental, and growth retardation. Discover the latest research on Nodding Syndrome here.

LRRK2 & Microtubules

Mutations in the LRRK2 gene are risk-factors for developing Parkinson’s disease (PD). LRRK2 mutations in PD have been shown to enhance its association with microtubules. Here is the latest research.

Related Papers

Current Protocols in Protein Science
Steven A Carr, Roland S Annan
Current Protocols in Protein Science
Guoan ZhangThomas A Neubert
Methods in Molecular Biology
David S Wishart
Journal of Digital Imaging
M A Goldberg
© 2021 Meta ULC. All rights reserved