Overview of peptide and protein analysis by mass spectrometry

Current Protocols in Protein Science
Steven A Carr, Roland S Annan

Abstract

One goal of this introductory overview is to try and dispel the still widely held, but now mistaken, belief that mass spectrometry is beyond the technical resources of the typical laboratory or facility engaged in the structural characterization or synthesis of peptides and proteins. Over the last five years, mass spectrometers and associated data systems have become available that can be operated by anyone capable of running a modern amino acid analyzer or Edman sequencer, yet are powerful enough to handle the most demanding analyses that the peptide or protein investigator might require. The remaining goals of this article are to familiarize peptide and protein chemists with the types of mass spectrometers that are appropriate for the majority of their analytical needs, to describe the kinds of experiments that can be performed with these instruments on a routine basis, and to discuss the kinds of information that these experiments provide. The emphasis here is on established tools and techniques that can realistically be used for problem solving. As a result, many useful instruments and techniques employed by the trained mass spectroscopist are not discussed here, because they do not satisfy these criteria or are not yet wid...Continue Reading

Citations

Sep 8, 2011·Current Protocols in Cell Biology·Sean GallagherJohn G R Hurrell
Mar 4, 2017·Current Protocols in Cell Biology·Duojiao NiSean Gallagher
Aug 2, 2016·Current Protocols in Immunology·Duojiao NiSean Gallagher
Oct 14, 2018·Current Protocols in Nucleic Acid Chemistry·Yu HiranoYasuo Komatsu
May 1, 2019·Current Protocols in Nucleic Acid Chemistry·Takaaki KurinomaruRyoji Kurita
Apr 4, 2017·Current Protocols in Protein Science·Duojiao NiSean Gallagher
Apr 25, 2008·Current Protocols in Immunology·Gregg B Fields
Jul 18, 2008·Current Protocols in Molecular Biology·Sean GallagherJohn G R Hurrell
Apr 3, 2016·Current Protocols in Molecular Biology·Duojiao NiSean Gallagher
Nov 20, 2008·Current Protocols in Immunology·Sean GallagherJohn G R Hurrell
Jun 1, 2010·Current Protocols in Chemical Biology·Eun Ryoung JangKyung Bo Kim

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