Ovicidal action of a crude enzymatic extract of the fungus Pochonia chlamydosporia against cyathostomin eggs

Veterinary Parasitology
F R BragaJosé Humberto de Queiroz

Abstract

The aims of this study were to test the action of the fungal extract of Pochonia chlamydosporia (VC4) on the hatching of cyathostomin eggs plated in Petri dishes containing 2% water-agar (2% WA) and its enzymatic activity in fecal cultures, in two experimental assays (A and B). The fungus P. chlamydosporia (VC4) was cultured in Erlenmeyer flasks (250ml) containing 50ml of liquid minimal medium supplemented with 0.2% gelatin for production of the crude enzymatic extract. Approximately 1kg of fresh feces was collected directly from the rectum of crossbred horses naturally infected with cyathostomins. The fecal material was used to obtain eggs and prepare fecal cultures. For assay A, one thousand eggs were plated on 4.5cm diameter Petri dishes together with 5ml of VC4 fungal filtrate and incubated at 26 degrees C in the dark for 24h. The control group consisted of 1000 eggs in Petri dishes containing 10ml of distilled water, which were incubated under the same conditions. After 24h, the total number of cyathostomin larvae present in each plate of the treated and control groups was counted. For assay B, about 20g of feces were added with 10ml of fungal extract of P. chlamydosporia (VC4) and incubated at 26 degrees C for 8 days. Thi...Continue Reading

References

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Citations

Jul 29, 2011·Veterinary Research Communications·Sebastião Rodrigo FerreiraAloízio Soares Ferreira
Jul 22, 2014·Acta Tropica·Rosane Teixeira LelisJackson Victor de Araújo
Aug 4, 2011·Equine Veterinary Journal. Supplement·C H StratfordJ B Matthews
Apr 30, 2020·Veterinary Sciences·Michela MaestriniStefania Perrucci
Oct 22, 2020·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Cristiane Telles BaptistaDaniela Isabel Brayer Pereira

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