Oviduct epithelium induces interferon-tau in bovine Day-4 embryos, which generates an anti-inflammatory response in immune cells.

Scientific Reports
Anup K TalukderAkio Miyamoto

Abstract

Recent studies indicate that communication between the bovine embryo and the mother begins in the oviduct. Here, we aimed to investigate the effect of embryos on bovine oviducts for their immune responses using an in vitro model. First, zygotes were cultured with or without bovine oviduct epithelial cells (BOECs) for 4 days, when embryos had reached the 16-cell stage. At that time, we detected interferon-tau (IFNT) in embryos co-cultured with BOECs, but not in embryos cultured alone. Next, peripheral blood mononuclear cells (PBMCs) were incubated either in media from embryo alone cultures or from co-cultures of embryos with BOECs. The medium from embryo alone cultures did not modulate PBMCs gene expression; whereas the embryo-BOEC co-culture medium increased interferon-stimulated genes (ISGs: ISG15, OAS1, MX2), STAT1, PTGES and TGFB1 but suppressed IL17 expression in PBMCs. Both IFNT-treated BOEC culture medium and IFNT-supplemented fresh medium alone without BOEC, modulated PBMCs gene expressions similar to those by the embryo-BOEC co-culture medium. Further, specific antibody to IFNT neutralized the effect of embryo-BOEC co-culture medium on PBMCs gene expression. Our results indicate that BOECs stimulate embryos to produce I...Continue Reading

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Citations

Oct 31, 2018·American Journal of Reproductive Immunology : AJRI·Anup K TalukderAkio Miyamoto
Jun 9, 2019·Scientific Reports·Mariana SponchiadoMario Binelli
Sep 14, 2019·Molecular Reproduction and Development·S Siddalingappa ArchanaStephen A Krawetz
Aug 15, 2020·Scientific Reports·Cecilia Constantino RochaGuilherme Pugliesi
Jan 27, 2021·Animals : an Open Access Journal From MDPI·Simone Tamara SchabmeyerMarie Margarete Meyerholz
Jan 25, 2020·Theriogenology·Beatriz Rodríguez-AlonsoDimitrios Rizos
Jan 30, 2021·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Keerthie DissanayakeAlireza Fazeli
Dec 23, 2020·Biomolecules·Leopoldo González-BrusiMaria Jiménez-Movilla
Feb 1, 2021·Animal Reproduction Science·Chi Sun YunNobuhiko Yamauchi
Feb 19, 2021·Biological Trace Element Research·Omid HamidiValiollah Palangi
Mar 23, 2021·Frontiers in Veterinary Science·Rosane MazzarellaJuliano Coelho da Silveira
Jun 15, 2021·Animal Reproduction·Cecilia Constantino RochaGuilherme Pugliesi
Jul 10, 2021·Journal of Animal Science·Laurel D QuirkeJennifer L Juengel

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Methods Mentioned

BETA
FCS
ELISA
flow cytometry
PCR

Software Mentioned

SPSS
ImageJ

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