Ovine aortic smooth muscle cells allow the replication of visna-maedi virus in vitro

Archives of Virology
C LerouxJean-François Mornex

Abstract

Visna-maedi virus induces in sheep an interstitial lung disease characterised by an accumulation of smooth muscle cells (SMC) or myomatosis. Infection by HIV-1 has been recently associated with disorders of the vessel-derived cells: primary pulmonary hypertension, coronary artery disease and smooth muscle tumors in humans. We hypothesized that, besides their regular targets (i.e. macrophages and lymphocytes), lentiviruses could infect smooth muscle cells. Smooth muscle cell cultures derived from ovine aorta were infected with visna-maedi virus strain K1514. The cultured cells were smooth muscle cells as demonstrated by their antigenic expression of alpha-actin and vimentin. The lentiviral infection of the smooth muscle cells was demonstrated by a typical cytopathic effect (syncytia), the expression of virus specific antigens, and the presence of genomic RNA detected by Northern blot analysis and RT PCR. The detection of a reverse transcriptase activity, the presence of viral RNA in supernatants of infected smooth muscle cells detected by RT PCR and their ability to infect ovine permissive fibroblasts demonstrated a productive infection. The ability of smooth muscle cells to be infected by lentiviruses may participate in the pat...Continue Reading

References

Jan 1, 1979·Physiological Reviews·J Chamley-CampbellR Ross
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A M SteffanA Kirn
Jun 1, 1992·The Journal of Pathology·A BaskervilleP J Greenaway
Aug 1, 1991·The Journal of General Virology·R G DalzielI McConnell
Nov 1, 1991·Journal of Virology·S E CraneJ E Clements
Dec 1, 1990·AIDS Research and Human Retroviruses·M P MoyerH E Gendelman
Aug 1, 1990·AIDS Research and Human Retroviruses·F PlataC Mayaud
Jul 15, 1990·The American Journal of Cardiology·W W GrodyW Lewis
May 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·S J WarnerP Libby
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D Zucker-Franklin, Y Z Cao
Jun 1, 1989·Journal of Virology·J M HarouseF Gonzalez-Scarano
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M TatenoJ A Levy
Jan 1, 1971·Veterinary Pathology·G Georgsson, P A Pálsson

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Citations

Aug 1, 1997·Journal of Medical Virology·T BerkeD O Matson
Nov 24, 1999·Journal of Medical Virology·Y JingX Jiang
Jan 30, 2007·Journal of Comparative Pathology·G D BrellouI Vlemmas
Sep 16, 2015·Veterinary Microbiology·E MinguijónR A Juste
Jan 1, 1996·Archives of Virology·D O MatsonA W Smith
Dec 3, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Gabrielle R MartinsMaria F da S Teixeira

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