Ovine liver proteome: Assessing mechanisms of seasonal weight loss tolerance between Merino and Damara sheep

Journal of Proteomics
Blake A MillerAndre M de Almeida

Abstract

The effect of feed restriction on the liver protein profiles of two different breeds of sheep was studied. We compared Merino with the Damara, breeds with respectively low and high tolerance to nutritional stress. Each breed was grouped into two nutritional treatments: restricted (12-14% loss of live weight) and control (maintenance). The trial lasted 42 days. Animals were sacrificed and liver samples subjected to label free shotgun proteomics. The resultant proteins had both their fold change and statistical significance in an unpaired t-test calculated to identify differential protein abundance. The tool WebGestalt was utilized to perform an Overrepresentation Enrichment Analysis (ORA) for gene ontology terms associated with the significant proteins. We further validated shotgun proteomics findings using a selected reaction monitoring (SRM)-based targeted proteomics approach, where similar trends in regulation were obtained for a subset of relevant proteins across an independent cohort of animals. Results confirm that Damara has adapted to nutritional stress by mobilizing stored fatty acids within adipose tissue and converting them to energy more efficiently than Merino. Finally, Merino had an overabundance pattern primarily ...Continue Reading

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Citations

Mar 14, 2020·Journal of Animal Physiology and Animal Nutrition·David Miguel RibeiroAndré Martinho de Almeida
Mar 27, 2019·Tropical Animal Health and Production·Lorenzo E Hernández-CastellanoAndré M de Almeida
Feb 6, 2020·Scientific Reports·Xinyue WangLi Zhang
Oct 13, 2019·Journal of Proteomics·Yongfu LaMingxing Chu
Apr 11, 2021·Journal of Proteomics·André M AlmeidaMaya Zachut

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