Ovis aries POU1F1 gene: cloning, characterization and polymorphism analysis

Genetica
Estela BastosRobert Renaville

Abstract

POU1F1 (PIT-1/GHF-1) is a transcription factor with critical role in the transcriptional regulation of multiple genes in the pituitary and also important for the survival, differentiation and proliferation of three pituitary cell types. To understand the regulation of POU1F1 gene in Ovis aries we report its cloning, sequencing and characterization. The sequenced 5787 bp included six exons and two complete introns. Ovine POU1F1 gene has a high level of conservation with its bovine, human and rat counterparts showing 98.2%, 91.2% and 86.2% of similarity at the coding level, respectively. All six exons were analyzed for polymorphism detection in 100 animals of the Portuguese indigenous ovine breed 'Churra da Terra Quente'. One polymorphism was found at codon 58 in exon 2, in one allele of 4 animals leading to a change from cysteine to tyrosine (2% allelic frequency). In exon 3 two polymorphisms were detected: a G to A transition altering a glycine to an asparagine at codon 89 in one allele of one animal (0.5% allelic frequency) and another G to A transition at codon 105 converting an alanine into a threonine in one allele of 3 animals (1.5% allelic frequency). These polymorphisms might change the structure of the POU1F1 protein an...Continue Reading

References

Jan 6, 1992·Biochimica Et Biophysica Acta·K TatsumiK Miyai
Nov 1, 1992·DNA and Cell Biology·E A WongM E el Halawani
May 1, 1995·Journal of Animal Science·T P YuM F Rothschild
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·S MajumdarH P Elsholtz
Dec 1, 1996·Journal of Molecular Endocrinology·J B LorensR Male
Mar 1, 1997·The Journal of Clinical Endocrinology and Metabolism·J T SchankeT G Golos
Nov 18, 1998·Cellular and Molecular Life Sciences : CMLS·M de la HoyaJ L Castrillo
Dec 22, 1999·Mammalian Genome : Official Journal of the International Mammalian Genome Society·I S Lantinga-van LeeuwenB A van Oost
Feb 22, 2000·Journal of Animal Science·J WoollardF A Ponce de León
Jan 29, 2002·Animal Reproduction Science·H S SunC K Tuggle
Mar 12, 2003·The Journal of Clinical Endocrinology and Metabolism·Yukiko HashimotoLaurie E Cohen
Jun 26, 2003·Nucleic Acids Research·Burkhard Rost, Jinfeng Liu
Aug 23, 2003·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Souzan SalemiPrimus E Mullis
Feb 28, 2004·Molecular Endocrinology·Gabriela NicaMatthias Hammerschmidt
Jun 25, 2004·Nucleic Acids Research·Burkhard RostJinfeng Liu

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Citations

Feb 9, 2012·Molecular Biology Reports·Maria Consuelo MuraVincenzo Carcangiu

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