Ovotransferrin is a matrix protein of the hen eggshell membranes and basal calcified layer

Connective Tissue Research
Joel GautronYves Nys

Abstract

The eggshell is an highly ordered structure deposited in the distal oviduct and composed of calcium carbonate and an organic matrix which is believed to influence its fabric. We have identified ovotransferrin as an 80 kDa matrix protein observed at high concentration in the uterine fluid at the initial stage of shell mineralization, by N-terminal sequencing and western blotting using monoclonal and polyclonal antibodies. It is present in extracts from demineralized eggshell and was localized by immunofluorescence in the eggshell membranes and mammillae, which are the sites of calcite nucleation. Northern blotting and RT-PCR demonstrated that ovotransferrin message was expressed in the proximal oviduct (magnum and white isthmus), and at a lower magnitude in the distal oviduct (red isthmus and uterus). Ovotransferrin was revealed by immunofluorescence in the tubular gland cells of the uterus. Calcium carbonate crystals grown in vitro in the presence of purified ovotransferrin showed large modifications of the calcite morphology. These observations and its presence in eggshell and membranes suggest a dual role for ovotransferrin, as a protein influencing nucleation and growth of calcite crystals and as a bacteriostatic filter to r...Continue Reading

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Citations

Jul 31, 2013·Animal Reproduction Science·Margherita MarzoniAntonio Felicioli
Sep 13, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·Karlheinz MannYves Nys
Nov 15, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Karlheinz MannMaxwell T Hincke
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Arthur D TinocoAnn M Valentine
Jul 15, 1999·British Poultry Science·S E Solomon
Aug 29, 2013·Proteome Science·Karlheinz Mann, Matthias Mann
Feb 8, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Maxwell T HinckeMarc D McKee
Jan 7, 2014·PloS One·Cristianne M M CordeiroMaxwell T Hincke
Jan 18, 2014·Proceedings of the National Academy of Sciences of the United States of America·Laura M HammPatricia M Dove
Dec 21, 2006·Poultry Science·A A SazanovT Malewski
May 20, 2009·Cellular and Molecular Life Sciences : CMLS·Megan L H Rose, Maxwell T Hincke
Mar 17, 2007·Connective Tissue Research·Ivan MiksíkKaterina Mikulikova
Jan 15, 2015·Journal of Proteomics·Megan Rose-MartelMaxwell T Hincke
Jan 15, 2008·Physiological and Biochemical Zoology : PBZ·Olivier Wellman-LabadieMaxwell T Hincke
May 1, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Young Wan HaYeong Shik Kim
Mar 9, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Jun XingMaxwell T Hincke
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Jan 13, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Karlheinz Mann, Frank Siedler
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Jun 19, 2012·Journal of Proteomics·Megan Rose-MartelMaxwell T Hincke
Jun 12, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Mahesh Kumar Sah, Subha Narayan Rath
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Oct 25, 2016·Journal of Proteomics·Charles Le PabicGilles Luquet
Jan 15, 2017·Journal of Proteomics·Tamer A E AhmedMaxwell T Hincke

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