Ovotransferrin is a matrix protein of the hen eggshell membranes and basal calcified layer

Connective Tissue Research
Joel GautronYves Nys

Abstract

The eggshell is an highly ordered structure deposited in the distal oviduct and composed of calcium carbonate and an organic matrix which is believed to influence its fabric. We have identified ovotransferrin as an 80 kDa matrix protein observed at high concentration in the uterine fluid at the initial stage of shell mineralization, by N-terminal sequencing and western blotting using monoclonal and polyclonal antibodies. It is present in extracts from demineralized eggshell and was localized by immunofluorescence in the eggshell membranes and mammillae, which are the sites of calcite nucleation. Northern blotting and RT-PCR demonstrated that ovotransferrin message was expressed in the proximal oviduct (magnum and white isthmus), and at a lower magnitude in the distal oviduct (red isthmus and uterus). Ovotransferrin was revealed by immunofluorescence in the tubular gland cells of the uterus. Calcium carbonate crystals grown in vitro in the presence of purified ovotransferrin showed large modifications of the calcite morphology. These observations and its presence in eggshell and membranes suggest a dual role for ovotransferrin, as a protein influencing nucleation and growth of calcite crystals and as a bacteriostatic filter to r...Continue Reading

References

Jul 1, 1992·British Poultry Science·Maxwell T HinckeR Narbaitz
Feb 1, 1982·European Journal of Biochemistry·J WilliamsK A Kuhn
Feb 1, 1982·European Journal of Biochemistry·J M Jeltsch, P Chambon
Jun 1, 1995·Calcified Tissue International·Maxwell T HinckeR Narbaitz
Feb 1, 1995·Matrix Biology : Journal of the International Society for Matrix Biology·T M WuA H Heuer
Dec 1, 1995·Matrix Biology : Journal of the International Society for Matrix Biology·M PinesA Bar
Apr 1, 1997·Matrix Biology : Journal of the International Society for Matrix Biology·M S FernándezJ L Arias
Jan 1, 1997·Connective Tissue Research·D A CarrinoA I Caplan
Jan 29, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·I LavelinM Pines
Aug 28, 1999·British Poultry Science·M PanheleuxYves Nys
Jun 30, 2000·Poultry Science·J M Dominguez-VeraYves Nys
Sep 12, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·Maxwell T HinckeYves Nys
Feb 27, 2001·Matrix Biology : Journal of the International Society for Matrix Biology·M Soledad FernandezJ L Arias
Jan 1, 1995·Connective Tissue Research·Maxwell T Hincke
Nov 1, 1997·Journal of Food Protection·Florence BaronGerard Brule

Citations

Jul 31, 2013·Animal Reproduction Science·Margherita MarzoniAntonio Felicioli
Sep 13, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·Karlheinz MannYves Nys
Nov 15, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Karlheinz MannMaxwell T Hincke
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Arthur D TinocoAnn M Valentine
Apr 15, 2008·British Poultry Science·Olivier Wellman-LabadieMaxwell T Hincke
Jul 15, 1999·British Poultry Science·S Solomon
Aug 29, 2013·Proteome Science·Karlheinz Mann, Matthias Mann
Feb 8, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Maxwell T HinckeMarc D McKee
Jan 7, 2014·PloS One·Cristianne M M CordeiroMaxwell T Hincke
Jan 18, 2014·Proceedings of the National Academy of Sciences of the United States of America·Laura M HammPatricia M Dove
Dec 21, 2006·Poultry Science·A A SazanovT Malewski
May 20, 2009·Cellular and Molecular Life Sciences : CMLS·Megan L H Rose, Maxwell T Hincke
Mar 17, 2007·Connective Tissue Research·Ivan MiksíkKaterina Mikulikova
Jan 15, 2015·Journal of Proteomics·Megan Rose-MartelMaxwell T Hincke
Jan 15, 2008·Physiological and Biochemical Zoology : PBZ·Olivier Wellman-LabadieMaxwell T Hincke
May 1, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Young Wan HaYeong Shik Kim
Mar 9, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Jun XingMaxwell T Hincke
Sep 15, 2004·Journal of Structural Biology·M S FernándezJ L Arias
Jan 13, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Karlheinz Mann, Frank Siedler
Sep 8, 2015·Journal of Proteomics·Cristianne M M Cordeiro, Maxwell T Hincke
Feb 12, 2009·FEMS Microbiology Reviews·Inne GantoisFilip Van Immerseel
Feb 22, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Olivier Wellman-LabadieMaxwell T Hincke
Jun 19, 2012·Journal of Proteomics·Megan Rose-MartelMaxwell T Hincke
Jun 12, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Mahesh Kumar Sah, Subha Narayan Rath
Oct 21, 2015·Journal of Agricultural and Food Chemistry·Sarbjeet MakkarNarayan C Rath
Sep 1, 2016·Journal of Proteomics·Charles Le PabicGilles Luquet
Jan 15, 2017·Journal of Proteomics·Tamer A E AhmedMaxwell T Hincke
Apr 1, 2020·Anatomia, histologia, embryologia·Mary-Cathrine Madekurozwa, Mike M Mpango
Dec 29, 2020·Poultry Science·Caroline Espejo StanquevisVittor Tuzzi Zancanela
Feb 14, 2021·BMC Molecular and Cell Biology·Joel GautronMaxwell T Hincke
Nov 5, 2020·International Journal of Molecular Sciences·Sissel B RønningMona E Pedersen
Apr 27, 2021·Frontiers in Cell and Developmental Biology·Anaïs Vitorino CarvalhoElisabeth Blesblois

Related Concepts

Metazoa
Body Fluids
Calcium
Chalk
Conalbumin
Polymorphism, Crystallization
Crystallography
Egg White Proteins
Egg Shell
Tissue Membrane

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Generating Insulin-Secreting Cells

Reprogramming cells or using induced pluripotent stem cells to generate insulin-secreting cells has significant therapeutic implications for diabetics. Here is the latest research on generation of insulin-secreting cells.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Epigenome Editing

Epigenome editing is the directed modification of epigenetic marks on chromatin at specified loci. This tool has many applications in research as well as in the clinic. Find the latest research on epigenome editing here.