Ovulation-selective genes: the generation and characterization of an ovulatory-selective cDNA library

The Journal of Endocrinology
A HourvitzN Dekel

Abstract

Ovulation-selective/specific genes, that is, genes preferentially or exclusively expressed during the ovulatory process, have been the subject of growing interest. We report herein studies on the use of suppression subtractive hybridization (SSH) to construct a 'forward' ovulation-selective/specific cDNA library. In toto, 485 clones were sequenced and analyzed for homology to known genes with the basic local alignment tool (BLAST). Of those, 252 were determined to be nonredundant. Of these 252 nonredundant clones, 98 were analyzed by probing mouse preovulatory and postovulatory ovarian cDNA. Twenty-five clones (26%) failed to show any signal, and 43 cDNAs tested thus far display a true ovulation-selective/specific expression pattern. In this communication, we focus on one such ovulation-selective gene, the fatty acid elongase 1 (FAE-1) homolog, found to be localized to the inner periantral granulosa and to the cumulus granulosa cells of antral follicles. The FAE-1 gene is a beta-ketoacyl-CoA synthase belonging to the fatty acid elongase (ELO) family, which catalyzes the initial step of very long-chain fatty acid synthesis. All in all, the present study accomplished systematic identification of those hormonally regulated genes t...Continue Reading

Citations

Jun 15, 2011·Journal of Assisted Reproduction and Genetics·Gil M YerushalmiAriel Hourvitz
Sep 13, 2011·The Journal of Biological Chemistry·François CollardEmile Van Schaftingen
Feb 27, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Ketty ShkolnikNava Dekel
May 16, 2014·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Eran BarzilayAriel Hourvitz
Sep 29, 2007·FEBS Letters·Ketty ShkolnikNava Dekel
Jul 27, 2010·Molecular and Cellular Endocrinology·Yuval YungAriel Hourvitz
Mar 8, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eran GershonNava Dekel

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