Ovum pick-up in sheep: a comparison between different aspiration devices for optimal oocyte retrieval

Reproduction in Domestic Animals = Zuchthygiene
C RodríguezP de Paz

Abstract

In vivo ovum pick-up (OPU) in sheep may be improved with a proper choice of aspiration elements (needle and tubing) and aspiration vacuum pressure. In the present study, two experiments were carried out. In Expt 1, visible follicles in ovaries of slaughtered ewes (treated separately according to their diameters: small<3 mm, medium 3-5 mm and large>5 mm) were aspirated using different combinations of the three studied factors such as aspiration flow rate (10, 20, 30, 40 and 50 ml water/min), needle gauge (18 and 20 G) and tubing inner diameter (1, 2 or 3 mm internal diameter). In Expt 2, a study with two 18 G needles of different lengths (18 G: 82 mm; 18 GL: 600 mm) was carried out, using ovaries obtained post-mortem, and performing in vivo laparoscopic follicular aspiration on ewes. We considered good quality oocytes as those with both complete compact cumulus and a homogeneous cytoplasm. Recovery rate, proportion of good quality oocytes (good quality oocytes/100 oocytes recovered) and overall efficiency (good quality oocytes/100 follicles aspirated) were noted. In Expt 1, aspiration flow rate affect remarkable proportion of good quality oocytes (69.5%, 50.5%, 44.8%, 36.5% and 28.3% for flows from 10 to 50 ml/min respectively, ...Continue Reading

References

Mar 29, 2000·Theriogenology·P J HendriksenS J Dieleman
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Citations

Mar 22, 2014·Theriogenology·Joanna Maria Gonçalves de Souza-FabjanPascal Mermillod
Jan 17, 2015·Iranian Red Crescent Medical Journal·Navid Dadashpour DavachiRazieh Moghadam
Jan 18, 2008·Reproduction in Domestic Animals = Zuchthygiene·K M MortonG Evans
Dec 10, 2013·Reproduction in Domestic Animals = Zuchthygiene·L C PadilhaW R R Vicente
Mar 3, 2015·Reproduction in Domestic Animals = Zuchthygiene·L F CrocomoS D Bicudo
Jan 10, 2020·Reproduction in Domestic Animals = Zuchthygiene·Felipe Farias Pereira da Câmara BarrosWilter Ricardo Russiano Vicente
Jun 19, 2019·Tropical Animal Health and Production·Gabriela Lisset Montes-QuirozHugo Bernal-Barragán
Apr 16, 2020·Animals : an Open Access Journal From MDPI·Jarosław WieczorekMirosław Cegła
Jan 16, 2021·Zygote : the Biology of Gametes and Early Embryos·Luciana Diniz RolaJosé Maurício Barbanti Duarte
Dec 5, 2017·Theriogenology·Claudinéia Silva MendesAna Lúcia Almeida Santana

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