Oxa1p mediates the export of the N- and C-termini of pCoxII from the mitochondrial matrix to the intermembrane space

FEBS Letters
K HellR A Stuart

Abstract

Oxa1p is a mitochondrial protein reported to be involved in the assembly of the cytochrome oxidase complex. In the absence of a functional Oxa1p, subunit II of the cytochrome oxidase accumulates as its precursor form (pCoxII). Using mitochondria isolated from a yeast strain bearing a temperature sensitive mutation in the Oxa1p, pet ts1402, we have analyzed the function of the Oxa1p protein. We demonstrate that the accumulation of pCoxII in the pet ts1402 mitochondria does not reflect a compromised Imp1p activity in this mutant. Furthermore, measurement of the membrane potential has shown it to be sufficient to support the export of CoxII from the matrix. Rather, we found that newly synthesized pCoxII accumulates in the matrix of the pet ts1402 mitochondria, because export across the inner membrane is inhibited in the pet ts1402 mitochondria. In conclusion, Oxa1p mediates the export of the N- and C-termini of the mitochondrially encoded subunit II of cytochrome oxidase from the matrix to the intermembrane space.

References

Nov 1, 1978·Cell·P Borst, L A Grivell
Jan 1, 1986·Annual Review of Biochemistry·A Tzagoloff, A M Myers
Mar 1, 1988·Molecular & General Genetics : MGG·M Schulze, G Rödel
Nov 10, 1995·The Journal of Biological Chemistry·J M HerrmannR A Stuart
Feb 24, 1995·The Journal of Biological Chemistry·F GärtnerN Pfanner
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·N BonnefoyG Dujardin
Dec 1, 1992·Trends in Cell Biology·R O PoytonG H Clarkson

❮ Previous
Next ❯

Citations

Jul 6, 2000·FEBS Letters·C M Koehler
Nov 5, 1999·Biochimica Et Biophysica Acta·W VoosN Pfanner
Apr 12, 2002·Gene·Antoni BarrientosAlexander Tzagoloff
Jun 30, 2000·Current Opinion in Cell Biology·R E DalbeyJ C Samuelson
Mar 24, 1999·Trends in Biochemical Sciences·R E Dalbey, C Robinson
Apr 16, 2009·Proceedings of the National Academy of Sciences of the United States of America·Soledad FunesJohannes M Herrmann
Jul 30, 2011·The Journal of Biological Chemistry·Mario H BarrosAlexander Tzagoloff
Apr 8, 2009·The Journal of Biological Chemistry·Takashi Sato, Katsuyoshi Mihara
Oct 28, 2009·Molecular and Cellular Biology·Flavia FontanesiAntoni Barrientos
Apr 25, 2007·Molecular and Cellular Biology·Heather L FiumeraThomas D Fox
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·H TjalsmaJ M van Dijl
Feb 1, 2007·Annual Review of Biochemistry·Walter Neupert, Johannes M Herrmann
Oct 14, 2000·Annual Review of Cell and Developmental Biology·R E Dalbey, A Kuhn
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Carla M Koehler
Jan 10, 2014·Human Molecular Genetics·Myriam BourensAntoni Barrientos
Feb 1, 2011·Annual Review of Biochemistry·Ross E DalbeyAndreas Kuhn
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·K HellR A Stuart
Feb 14, 2015·Journal of Molecular Biology·Walter Neupert
Feb 13, 2003·Molecular Microbiology·Linda FröderbergJan-Willem L De Gier

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.