PMID: 8583575Mar 1, 1996Paper

Oxalate toxicity in LLC-PK1 cells, a line of renal epithelial cells

The Journal of Urology
C ScheidM Menon

Abstract

The present studies assessed the possibility that high concentrations of oxalate may be toxic to renal epithelial cells. Subconfluent cultures of LLC-PK1 cells were exposed to oxalate, and the effects on cell morphology, membrane permeability to vital dyes, DNA integrity and cell density were assessed. Oxalate exposure produced time- and concentration-dependent changes in the light microscopic appearance of LLC-PK1 cells with higher concentrations ( > 140 microM.) inducing marked cytosolic vacuolization and nuclear pyknosis. Exposure to oxalate also increased membrane permeability to vital dyes, promoted DNA fragmentation and, at high concentrations (350 microM. free oxalate), induced a net loss of LLC-PK1 cells. Since high concentrations of oxalate can be toxic to renal epithelial cells, hyperoxaluria may contribute to several forms of renal disease including both calcium stone disease and end-stage renal disease.

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Citations

Dec 3, 1999·Biochemical and Biophysical Research Communications·R VijayaR Selvam
Jan 1, 1997·World Journal of Urology·C F VerkoelenJ C Romijn
Jul 14, 2004·Urological Research·Kemal SaricaRamazan Uçak
Jul 6, 2004·Urological Research·Katsuhito MiyazawaShogo Katsuda
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Dec 1, 2005·Urological Research·Fernando C DelvecchioGlenn M Preminger
Aug 23, 2006·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Ipek AkilSevda Müftüoğlu
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Sep 25, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Naoto SassaMomokazu Gotoh
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Feb 14, 2008·International Journal of Urology : Official Journal of the Japanese Urological Association·Masao Tsujihata

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