PMID: 11334358May 4, 2001Paper

Oxalic acid stabilizes dopamine, serotonin, and their metabolites in automated liquid chromatography with electrochemical detection

Journal of Chromatography. B, Biomedical Sciences and Applications
Aino KankaanpääTimo Seppälä

Abstract

Use of antioxidative agents is required in automated LC assay of microdialysis samples, due to rapid degradation of the monoamine neurotransmitters and their metabolites. Addition of oxalic acid prevented degradation of dopamine, serotonin, 3,4-dihydroxyphenylacetic acid, homovanillic acid and 5-hydroxyindoleacetic acid efficiently: after a 24-h incubation at room temperature the decreases in peak heights were less than 10%. The long-term stability of the analytes, however, was still enhanced when acetic acid and L-cysteine were included in the solution. Using this antioxidative solution, the monoamine neurotransmitters and their metabolites could be determined with an automated LC assay even at room temperature.

Citations

Feb 23, 2007·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Tomi RantamäkiEero Castrén
Feb 18, 2009·Genes, Brain, and Behavior·Paul J MeyerTamara J Phillips
Sep 4, 2004·Genes, Brain, and Behavior·O Y VekovischevaE R Korpi
Aug 10, 2015·Neuropharmacology·Anne-Julie Chabot-DoréLaura S Stone
Jan 28, 2014·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Eric W CrickCraig E Lunte
Jul 16, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jolien Van SchoorsAnn Van Eeckhaut
May 29, 2004·Peptides·Eagle Yi-Kung HuangPao-Luh Tao
Jan 24, 2002·The Journal of Pharmacology and Experimental Therapeutics·Aino KankaanpääTimo Seppälä
Dec 7, 2018·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·R A NolanP J Gaskill
Jul 27, 2018·JCI Insight·Adam M BelangerNelson S Yew
Oct 12, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·A T NguyenP P De Deyn

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