Oxanine DNA glycosylase activity from Mammalian alkyladenine glycosylase.

The Journal of Biological Chemistry
Thomas M HitchcockWeiguo Cao

Abstract

Oxanine (Oxa) is a deaminated base lesion derived from guanine in which the N(1)-nitrogen is substituted by oxygen. This work reports the mutagenicity of oxanine as well as oxanine DNA glycosylase (ODG) activities in mammalian systems. Using human DNA polymerase beta, deoxyoxanosine triphosphate is only incorporated opposite cytosine (Cyt). When an oxanine base is in a DNA template, Cyt is efficiently incorporated opposite the template oxanine; however, adenine and thymine are also incorporated opposite Oxa with an efficiency approximately 80% of a Cyt/Oxa (C/O) base pair. Guanine is incorporated opposite Oxa with the least efficiency, 16% compared with cytosine. ODG activity was detected in several mammalian cell extracts. Among the known human DNA glycosylases tested, human alkyladenine glycosylase (AAG) shows ODG activity, whereas hOGG1, hNEIL1, or hNEIL2 did not. ODG activity was detected in spleen cell extracts of wild type age-matched mice, but little activity was observed in that of Aag knock-out mice, confirming that the ODG activity is intrinsic to AAG. Human AAG can excise Oxa from all four Oxa-containing double-stranded base pairs, Cyt/Oxa, Thy/Oxa, Ade/Oxa, and Gua/Oxa, with no preference to base pairing. Surprising...Continue Reading

References

Oct 1, 1992·Chemical & Pharmaceutical Bulletin·H KamiyaA Matsukage
Oct 9, 1980·Nature·B K Duncan, J H Miller
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·M Saparbaev, J Laval
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·B HangR H Elder
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·B P EngelwardL D Samson
Feb 28, 1998·Nucleic Acids Research·G C HoopsV J Davisson
Mar 5, 1999·Mutation Research·S BurneyS R Tannenbaum
Aug 10, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M D WyattL D Samson
Dec 3, 1999·Science·T Lindahl, R D Wood
Dec 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·A Y LauT Ellenberger
Jun 28, 2002·Free Radical Biology & Medicine·Susan S Wallace
Feb 1, 2003·Nucleic Acids Research·Viengsai VongchampaPeter Dedon
Dec 23, 2003·The Journal of Biological Chemistry·Patrick J O'Brien, Tom Ellenberger

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Citations

Aug 13, 2010·Biochemistry·Michael R Baldwin, Patrick J O'Brien
Oct 9, 2008·Biochemistry·Mark Hedglin, Patrick J O'Brien
Feb 9, 2006·Chemical Reviews·Patrick J O'Brien
Jan 14, 2009·Proceedings of the National Academy of Sciences of the United States of America·Lisiane B MeiraLeona D Samson
Jun 16, 2010·The Journal of Biological Chemistry·Derek M Lyons, Patrick J O'Brien
Aug 4, 2004·Nucleic Acids Research·Thomas M HitchcockWeiguo Cao
Oct 13, 2005·Nucleic Acids Research·Seung Pil PackKeisuke Makino
Nov 1, 2011·Nucleic Acids Research·Seung Pil PackKenji Kanaori
Oct 18, 2012·Nucleic Acids Research·Michael R Baldwin, Patrick J O'Brien
Mar 4, 2005·Biological & Pharmaceutical Bulletin·Mika HoriHiroyuki Kamiya
Feb 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sreenivas KanugulaAnthony E Pegg
Oct 18, 2014·Nucleic Acids Research·Jenna M Hendershot, Patrick J O'Brien
Dec 25, 2012·Cellular and Molecular Life Sciences : CMLS·Weiguo Cao
Jun 6, 2012·Mutation Research·Rongjuan MiWeiguo Cao
Oct 24, 2007·DNA Repair·Liang DongWeiguo Cao
Nov 1, 2015·Nucleic Acids Research·Nicholas C BauerPaul W Doetsch
Dec 17, 2014·Biochemistry·Mark HedglinPatrick J O'Brien
Mar 21, 2008·Chembiochem : a European Journal of Chemical Biology·Hauh-Jyun Candy ChenSiou-Siou Yang
Jul 11, 2013·The Journal of Biological Chemistry·Mark HedglinPatrick J O'Brien
Jan 12, 2005·Biochemistry·Hong FengWeiguo Cao
Jun 26, 2018·Biochemistry·Erin L TaylorPatrick J O'Brien

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