PMID: 7538657May 1, 1995Paper

Oxantel-activated single channel currents in the muscle membrane of Ascaris suum

Parasitology
V M Dale, Richard J Martin

Abstract

The patch clamp technique was used to investigate the action of the anthelmintic drug, oxantel, on nicotinic acetylcholine receptor (nAChR) currents recorded from vesicles of the somatic muscle cells of the nematode parasite Ascaris suum. The amplitudes of the currents were analysed at different membrane potentials to determine the single channel conductance. Also the open and closed durations were measured to determine the kinetic properties of the activated channel. Oxantel activated single nAChR currents throughout a concentration range 10-100 microM, these currents were not observed with oxantel-free pipette solutions. The mean open time of the activated channels at a membrane potential of -75 mV and a concentration of 10 microM was 1.34 ms. At higher concentrations the open times were shorter and voltage sensitive, decreasing in duration on hyperpolarization, thus suggesting open channel block. The kinetics were analysed using a simple channel block model. The forward block rate, K + B, increased with increasing oxantel concentration but showed little increase as the membrane was hyperpolarized. K + B was 2.41 x 10(7) M-1 s-1 at -50 mV and 2.64 x 10(7) M-1 s-1 at -100 mV. The unblocking rate constant, K-B, did exhibit volt...Continue Reading

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Citations

Jun 14, 2005·International Journal for Parasitology·Mark M LevandoskiRichard J Martin
Feb 14, 2014·The New England Journal of Medicine·Benjamin SpeichJennifer Keiser
Jan 1, 1996·Parasitology·R J MartinI Murray
Jul 1, 1997·The Veterinary Journal·R J Martin
Nov 14, 2007·Invertebrate Neuroscience : in·Alan P Robertson, Richard J Martin
Jan 13, 2010·Molecular and Biochemical Parasitology·Sreekanth PuttacharyRichard J Martin
Jan 22, 2008·International Journal for Parasitology·Sasa M TrailovicRichard J Martin
Feb 9, 2018·Acta veterinaria·Melanie AbongwaAlan P Robertson

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