Oxcarbazepine-loaded polymeric nanoparticles: development and permeability studies across in vitro models of the blood-brain barrier and human placental trophoblast

International Journal of Nanomedicine
Antonio LopalcoErik Rytting

Abstract

Encapsulation of antiepileptic drugs (AEDs) into nanoparticles may offer promise for treating pregnant women with epilepsy by improving brain delivery and limiting the transplacental permeability of AEDs to avoid fetal exposure and its consequent undesirable adverse effects. Oxcarbazepine-loaded nanoparticles were prepared by a modified solvent displacement method from biocompatible polymers (poly(lactic-co-glycolic acid) [PLGA] with or without surfactant and PEGylated PLGA [Resomer(®) RGPd5055]). The physical properties of the developed nanoparticles were determined with subsequent evaluation of their permeability across in vitro models of the blood-brain barrier (hCMEC/D3 cells) and human placental trophoblast cells (BeWo b30 cells). Oxcarbazepine-loaded nanoparticles with encapsulation efficiency above 69% were prepared with sizes ranging from 140-170 nm, polydispersity indices below 0.3, and zeta potential values below -34 mV. Differential scanning calorimetry and X-ray diffraction studies confirmed the amorphous state of the nanoencapsulated drug. The apparent permeability (Pe ) values of the free and nanoencapsulated oxcarbazepine were comparable across both cell types, likely due to rapid drug release kinetics. Transport...Continue Reading

Citations

Jan 19, 2018·Materials·Elena Fernández FernándezCatherine M Greene
Mar 31, 2018·Scientific Reports·Leonie AengenheisterTina Buerki-Thurnherr
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Elías QuezadaDolores Viña
Dec 12, 2017·Molecular Pharmaceutics·Maria Laura PatiNunzio Denora
Aug 7, 2017·Molecular Pharmaceutics·Tommaso CassanoCosimo D Altomare

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Methods Mentioned

BETA
dynamic light scattering
differential scanning calorimetry
X-ray
differential scanning

Software Mentioned

DigitalMicrograph
FIT2D
[UNK]
Universal Analysis 2000

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