Oxidant and anti-oxidant effects on arachidonate metabolism by rabbit ocular tissues

Journal of Ocular Pharmacology
J S HurstC S Short

Abstract

Ocular tissues have the capacity to metabolize arachidonate to prostaglandins and related materials, such as hydroxy arachidonate derivatives (HETEs) which are potent mediators of the inflammatory response. Reactive oxygen species are also present during the inflammatory response, resulting in an altered oxidative environment within the eye. This study was designed to evaluate the possible impact of the oxidant hydrogen peroxide and anti-oxidants, ascorbic acid and glutathione, upon arachidonate metabolism. It was found that hydrogen peroxide was observed to potently inhibit arachidonate metabolism in the cornea, but not in the iris-ciliary body. This might be related to a more efficient detoxification of hydrogen peroxide by iris-ciliary body. Ascorbate appeared to have a general stimulatory influence upon arachidonate metabolism in the iris-ciliary body. In the cornea, ascorbate selectively reduced metabolism to HETE while enhancing the products generated by the cyclo-oxygenase pathway. In both cornea and iris-ciliary body reduced glutathione suppressed conversion of arachidonate to its active metabolites. These observations indicate that arachidonate metabolism in ocular tissues is sensitive to the oxidative environment.

References

Sep 30, 1975·Annals of the New York Academy of Sciences·B H BielskiP C Chan
Apr 1, 1979·Archives of Biochemistry and Biophysics·M E HemlerW E Lands
Sep 8, 1978·Science·I Fridovich
Jan 13, 1977·Nature·F A KuehlC G Van Arman
Sep 1, 1977·Prostaglandins·F CotteeJ R Vane
Dec 1, 1985·Experimental Eye Research·R N WilliamsC A Paterson
Jul 1, 1986·Experimental Eye Research·R N Williams, C A Paterson
Nov 28, 1980·Science·F A Kuehl, R W Egan

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.