PMID: 9165296Jan 1, 1997Paper

Oxidant generation with K(+)-induced depolarization in the isolated perfused lung

Free Radical Biology & Medicine
A B Al-MehdiA B Fisher

Abstract

This study evaluated whether cell membrane depolarization can induce oxidant generation in the isolated perfused rat lung as has been demonstrated with bovine pulmonary artery endothelial cells. Depolarization was produced by perfusing the lungs with high [K+] or with glyburide and was evaluated with bis-oxonol lung surface fluorometry. Lung surface bis-oxonol fluorescence increased above baseline (at 5.9 mM K+) by 18.5% with 24 mM K+, 35% with 48 mM K+, and 67% with 96 mM K+, indicating graded membrane depolarization, and by 75% during perfusion with 10 microM glyburide. Oxidant generation was evaluated with hydroethidine lung surface fluorometry, and with assay of tissue thiobarbituric acid reactive substance (TBARS), conjugated dienes, and perfusate H2O2. Depolarization by high K+ or glyburide led to significant increases in generation of tissue oxidants and lipid peroxidation. Bodipy-FL-glyburide microfluorography showed localization of glyburide binding primarily to vascular endothelial cells vascular and airway smooth muscle cells, alveolar type II cells, and to nonciliated cells of the airway epithelium. These results indicate that cellular depolarization is associated with oxidant generation by the lung and suggests a r...Continue Reading

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Citations

Nov 5, 2008·Cell Biochemistry and Biophysics·Shampa ChatterjeeAron B Fisher
Dec 2, 2000·European Journal of Pharmacology·V E MendizabalE Adler-Graschinsky
May 11, 2002·Critical Care Medicine·Aron B FisherYefim Manevich
Aug 10, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Julie NijmehElizabeth M Wagner
Jun 6, 1998·American Journal of Respiratory Cell and Molecular Biology·A B Al-MehdiA B Fisher
Nov 15, 2011·Annual Review of Physiology·Elizabeth A BrowningAron B Fisher
Aug 12, 2015·Current Opinion in Organ Transplantation·Mary K PorteousJason D Christie
Nov 17, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Geoffrey P Dobson
Aug 28, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Ikuo MatsuzakiAron B Fisher
Jun 27, 2003·American Journal of Physiology. Cell Physiology·Shampa ChatterjeeAron B Fisher
Apr 12, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Shampa ChatterjeeAron B Fisher
May 1, 2020·Frontiers in Bioengineering and Biotechnology·N Valerio Dorrello, Gordana Vunjak-Novakovic
Sep 23, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Shampa ChatterjeeAron B Fisher
Oct 23, 2001·American Journal of Physiology. Heart and Circulatory Physiology·Z WeiA B Fisher
Sep 15, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·C SongA B Fisher
Aug 19, 2005·American Journal of Physiology. Cell Physiology·Tatyana MilovanovaAron B Fisher
Mar 7, 2020·Journal of Applied Physiology·Said H AudiElizabeth R Jacobs
Oct 23, 2002·Journal of Applied Physiology·Arijit RoySukhamay Lahiri
May 25, 2021·Seminars in Respiratory and Critical Care Medicine·Jake G Natalini, Joshua M Diamond

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