Oxidant inhibition of epithelial active sodium transport

Free Radical Biology & Medicine
S MatalonB A Freeman

Abstract

The purpose of this study was to quantify the effects of extracellularly generated partially reduced oxygen species on active sodium (Na+) transport across the ventral toad skin, a well-studied epithelium. Sections of skin from decapitated toads were mounted in an Ussing chamber, bathed on both sides with electrolyte solution containing 500 microM xanthine and bubbled continuously with room air. The tissues were short-circuited, and short-circuit current (Isc) and tissue resistance (Rt) were monitored continuously with an automatic voltage clamp apparatus. Fifteen mU/ml of xanthine oxidase (XO), either purchased from Calbiochem or purified from cream, were instilled in either the apical (mucosal) or basolateral (serosal) baths at t = 0 and t = 10 min. Hydrogen peroxide (H2O2) concentrations increased to 200 microM within the first 20 min and then decreased, reaching a value of 40 microM by 60 min. Mean [H2O2] was 90 microM. Instillation of XO in the apical bath resulted in a large decrease in Isc and an increase in Rt, their values being 43% and 160% of their corresponding controls 85 min after the first instillation. Addition of superoxide dismutase and catalase completely prevented these changes. Instillation of XO in the bas...Continue Reading

References

Aug 1, 1975·Archives of Biochemistry and Biophysics·W R WaudK V Rajagopalan
Aug 1, 1985·The Journal of General Physiology·G K WangD C Eaton
Sep 1, 1985·The Journal of Clinical Investigation·M J WelshR M Husted
Sep 1, 1984·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·B E GoodmanE D Crandall
Jul 1, 1980·Physiological Reviews·A D MacknightA Leaf
Apr 1, 1981·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·S Matalon, E A Egan
Aug 1, 1981·The Journal of Physiology·A Luger, K Turnheim
Jul 1, 1980·Circulation Research·S WallensteinJ L Fleiss

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Citations

Aug 17, 1990·Biochimica Et Biophysica Acta·S MatalonB A Freeman
Dec 1, 1995·Free Radical Biology & Medicine·G D Buffinton, W F Doe
May 20, 1998·Free Radical Biology & Medicine·M MacchioneP H Saldiva
May 29, 1998·American Journal of Respiratory and Critical Care Medicine·J R MichaelR W Day
Feb 19, 2002·American Journal of Respiratory and Critical Care Medicine·Phillip FactorVidas Dumasius
Jul 1, 1990·The Journal of Clinical Investigation·S S KarayalcinD W Powell
Feb 17, 1992·Biochimica Et Biophysica Acta·M L BauerS Matalon
Oct 29, 2000·Journal of Applied Physiology·P FactorJ I Sznajder
Mar 23, 2010·Expert Review of Respiratory Medicine·Karen E IlesSadis Matalon
Nov 14, 1998·The American Journal of Physiology·M D DuVallS Matalon

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