Oxidant stress following renal ischemia: changes in the glutathione redox ratio

Kidney International
R N McCoyR F Burk

Abstract

Pretreatment of animals with certain antioxidant enzymes and substances decreases renal damage following ischemia and reperfusion. The hypothesis that reoxygenation imposes an oxidant stress has been used to explain this. The present study has directly assessed oxidant stress under these conditions by measuring the glutathione redox ratio ([GSSG/(GSH + GSSG)] x 100) in freeze-clamped kidney. The glutathione peroxidase system plays a role in removing peroxides which result from oxidant stress, generating GSSG from GSH in the process. The selenium-dependent glutathione peroxidase can metabolize H2O2 and other hydroperoxides. A non-selenium-dependent glutathione peroxidase activity is present and can metabolize organic hydroperoxides, but it cannot metabolize H2O2. Under anesthesia, the left renal artery was occluded for 40 minutes and then reflow was allowed. Kidneys were freeze clamped before reflow and after 5, 10, and 15 minutes of reflow. The contralateral kidney was freeze clamped and used as a control. The control value for the glutathione redox ratio was 1.09 +/- 0.05. This fell during ischemia to 0.67 +/- 0.22 and increased significantly to 1.66 +/- 0.29 after five minutes of reperfusion. By 15 minutes it had returned to ...Continue Reading

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·O W Griffith, A Meister
May 23, 1976·Biochemical and Biophysical Research Communications·J R Prohaska, H E Ganther
Jan 17, 1985·The New England Journal of Medicine·J M McCord
Jan 1, 1987·Methods in Enzymology·M F LouD M Ziegler
Jan 1, 1987·Methods in Enzymology·R F Burk
May 1, 1985·Journal of Neurochemistry·S YoshidaM D Ginsberg
Jan 1, 1970·British Journal of Industrial Medicine·D G Clark, E W Hurst
Oct 1, 1984·The Journal of Clinical Investigation·M S PallerT F Ferris

❮ Previous
Next ❯

Citations

Nov 1, 1991·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·S P Andreoli
Dec 29, 1995·European Journal of Pharmacology·R ParoniR Trazzi
Jan 1, 1990·Pharmacology & Therapeutics·X Q ShanD P Jones
May 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·R CamposA Valenzuela
Jan 1, 1991·Free Radical Biology & Medicine·J K BeckmanH L Greene
Aug 1, 1993·Free Radical Biology & Medicine·M L BarnardJ F Turrens
May 8, 2004·Experimental Gerontology·Maria Heloisa Massola ShimizuAntonio Carlos Seguro
Jan 21, 2004·Nitric Oxide : Biology and Chemistry·Frederick A Gage, Yoram Vodovotz
Dec 2, 2000·The American Journal of Medicine·K A Nath, S M Norby
Dec 16, 2000·Clinical and Experimental Pharmacology & Physiology·R JiménezM E Muñoz
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S R Abul-EzzS V Shah
Oct 30, 2007·Evidence-based Complementary and Alternative Medicine : ECAM·Meenal KumarAshok Kumar
Feb 1, 1990·The Journal of Clinical Investigation·L J MandelW R Jacobs
Dec 1, 1991·The Journal of Clinical Investigation·A K SalahudeenK A Nath
Feb 6, 2004·Best Practice & Research. Clinical Anaesthesiology·Norbert H Lameire, Raymond Vanholder
Mar 1, 1991·Kidney International·J M Weinberg
Dec 1, 1990·Kidney International·K A Nath, M S Paller
Aug 1, 1990·Kidney International·T YoshiokaI Ichikawa
Dec 1, 1991·Kidney International·M S Paller, T V Neumann
Dec 1, 2014·The Journal of Thoracic and Cardiovascular Surgery·Szu-Yuan LiLin Shing-Jong
Jul 28, 2009·The Annals of Thoracic Surgery·Umberto BenedettoRiccardo Sinatra
Jan 1, 1992·Renal Failure·J M Weinberg
Jan 31, 2008·Journal of Intensive Care Medicine·Mitchell H RosnerMark D Okusa
Aug 21, 2007·Clinical Journal of the American Society of Nephrology : CJASN·Mitchell H Rosner, Mark D Okusa
Nov 8, 2014·Clinical Journal of the American Society of Nephrology : CJASN·Robert H ThieleMitchell H Rosner
Feb 1, 1996·The British Journal of Surgery·S C WeightM L Nicholson
Nov 28, 2012·Journal of Anesthesia·Satyen Parida, Ashok Shankar Badhe
Dec 11, 2002·Renal Failure·Angel Fernández-FúnezLucia Zafrilla

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.