Oxidant stress inhibits the endogenous production of lipoxygenase metabolites in rat lungs and fish gills

Free Radical Biology & Medicine
J B German, M L Hu

Abstract

Hydroperoxides are potent initiators of lipid peroxidation in vivo. Acyl hydroperoxides may also regulate various aspects of lipid metabolism. In this study we investigated the regulation of the endogenous 12 lipoxygenase in trout gill and rat lung, a prominent acyl hydroperoxide catalyst in these tissues. Initial experiments revealed that the enzyme from trout gill was activated by hydroperoxides at low levels and inactivated by the same hydroperoxides at high levels. Homogenization of these tissues resulted in the production of a predominant metabolite class from released endogenous polyunsaturated fatty acids, the 12 lipoxygenase products. In rat lung, arachidonic acid was the major polyunsaturated fatty acid released and 12 (S) HETE was the major metabolite. In trout gill 20:4, 20:5 n3, and 22:6 n3 were released and the 12(S), 12, and 14 hydroxy derivatives the corresponding metabolites. Computer simulations of the sensitivity of these enzymes to hydroperoxides predicted that exogenous oxidant stress would reduce significantly the production of HETEs. Tertiary butyl hydroperoxide was added to tissue homogenates and resulted in elimination of greater than 95% of the lipoxygenase activity. These results suggest that the lipox...Continue Reading

References

Apr 1, 1979·Archives of Biochemistry and Biophysics·M E HemlerW E Lands
Jan 1, 1989·Free Radical Biology & Medicine·A L TappelC G Fraga
Feb 7, 1983·Biochimica Et Biophysica Acta·C YokoyamaC R Pace-Asciak
Apr 1, 1984·Journal of Inorganic Biochemistry·J Chaudiere, A L Tappel

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Citations

Dec 1, 1992·Calcified Tissue International·R Bracci
Feb 1, 1992·The International Journal of Biochemistry·A P KulkarniI S Richards
May 21, 1999·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·J W HollandA F Rowley
Jun 1, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Catherine SeguineauPhilippe Soudant
Dec 12, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ming Long Sirius ChungChung-Yung Jetty Lee
Sep 19, 2018·Neuropsychology, Development, and Cognition. Section B, Aging, Neuropsychology and Cognition·Ranjini Mohan, Christine Weber
Mar 31, 2004·Language, Speech, and Hearing Services in Schools·Nancy E Hall

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