PMID: 11922914Mar 30, 2002Paper

Oxidant stress mechanism of homocysteine potentiating Con A-induced proliferation in murine splenic T lymphocytes

Cardiovascular Research
Qin ZhangXian Wang

Abstract

An elevated plasma homocysteine (Hcy) level is considered an independent risk factor for atherosclerosis. However, the mechanisms by which hyperhomocysteinemia induces atherosclerosis are only partially understood. The effect of Hcy on T lymphocyte proliferation and its mechanisms were examined in normal and hyperhomocysteinemia ApoE-knockout mice. The mouse splenic T-cells were treated with Hcy, related compounds and/or antioxidants in the presence or absence of Concanavalin A (Con A). DNA synthesis, cell apoptosis, interleukin-2 level and production of reactive oxygen species (ROS) were measured. Hcy (0.3-3.0 mM) and related compounds with thiol (-SH), such as cysteine and glutathione significantly potentiated Con A-induced proliferation and partially inhibited apoptosis in T lymphocytes, but it had no direct effect on resting T lymphocyte. ApoE-knockout mice with hyperhomocysteinemia (the level of plasma Hcy was 20.3+/-2.9 vs. 2.6+/-0.6 microM in control group, P<0.05) had a significant promotion of T-cell proliferation in response to Con A. Hcy (0.3-3.0 mM) also increased the intracellular ROS. Radical scavengers reduced Hcy effect. These data indicate that ROS generated by thiol (-SH) of Hcy auto-oxidation are involved in ...Continue Reading

Citations

Sep 4, 2012·Antioxidants & Redox Signaling·Pravin KesarwaniShikhar Mehrotra
Apr 21, 2004·European Journal of Biochemistry·Anna MangiagalliFabio Blandini
Feb 27, 2007·International Immunopharmacology·Xiaoping YangMoucheng Wu
Feb 6, 2007·International Immunopharmacology·Xiaoping YangMoucheng Wu
Jul 12, 2016·Canadian Journal of Physiology and Pharmacology·Sudhakar VeerankiSuresh C Tyagi
Apr 8, 2017·Molecular and Cellular Biochemistry·Anastasia FamiltsevaSuresh C Tyagi
Aug 25, 2017·British Journal of Pharmacology·Yi FuWei Kong
Sep 8, 2004·Journal of Cellular Biochemistry·Hideo TsuboiIzumi Nakashima
Oct 29, 2005·Bulletin of Experimental Biology and Medicine·A A Boldyrev
Feb 22, 2012·Biochemistry. Biokhimii︠a︡·A A BoldyrevE A Vladychenskaya
Nov 2, 2019·Bioscience Reports·Xiran LinZhiqi Song
Jun 20, 2020·Dermatologic Therapy·Yogendra SinghKamal Dua

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