Oxidant stress reduces insulin responsiveness in 3T3-L1 adipocytes

The American Journal of Physiology
A RudichN Bashan

Abstract

Increased oxidant stress has been suggested to occur in diabetes and to contribute to the development of late diabetic complications. Whether oxidant stress plays a role in the development or progression of insulin resistance is not known. In this study we hypothesized that exposing 3T3-L1 adipocytes to prolonged micromolar concentrations of H2O2 would reduce their acute metabolic responses to insulin stimulation. 3T3-L1 adipocytes exposed to 25 mU/ml glucose oxidase (GO) for 18 h exhibited a threefold increase in basal 2-deoxyglucose (2-DG) uptake activity. However, net increase in 2-DG uptake activity after acute insulin (100 nM) stimulation was 355 +/- 56 pmol.mg protein-1.min-1 in control vs. 198 +/- 41 pmol.mg protein-1.min-1 in GO-pretreated cells (P < 0.05). Basal lipogenesis activity was significantly enhanced by GO, but acute insulin stimulation resulted in significantly reduced lipogenesis activity (29 +/- 4 vs. 11 +/- 1 nmol glucose/well for control and 50 mU/ml GO, respectively, P = 0.001). Glycogen synthase alpha activity was reduced by GO (78 +/- 1 vs. 43 +/- 2 pmol UDP-glucose.mg protein-1.min-1, P = 0.03), whereas insulin stimulation of glycogen synthase was reduced, exhibiting a right shift in the insulin dose-...Continue Reading

Citations

Dec 13, 2003·The Journal of Clinical Endocrinology and Metabolism·Xinhua ChenT Peter Stein
Jul 24, 2003·Experimental Biology and Medicine·Carla D GardnerEvangeline D Motley
Dec 20, 2007·American Journal of Physiology. Endocrinology and Metabolism·Betsy B DokkenErik J Henriksen
Nov 27, 2008·American Journal of Physiology. Endocrinology and Metabolism·Julia KovsanAssaf Rudich
Aug 23, 2007·American Journal of Physiology. Endocrinology and Metabolism·Edward R DuncanMark T Kearney
Jun 16, 2001·Journal of Applied Physiology·V SaengsirisuwanE J Henriksen
Jun 24, 2008·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·S H Ki, S G Kim
Oct 30, 2008·American Journal of Physiology. Endocrinology and Metabolism·Annalisa NatalicchioFrancesco Giorgino
Jul 14, 2010·Antioxidants & Redox Signaling·Xin Gen Lei, Marko Z Vatamaniuk
Feb 28, 2015·Oxidative Medicine and Cellular Longevity·Niara da Silva MedeirosCaroline Dani
Jan 27, 2018·Biological Chemistry·Pyotr A Tyurin-KuzminAlexander V Vorotnikov
Jan 19, 2011·Biochemical and Biophysical Research Communications·Maggie K Diamond-StanicErik J Henriksen
May 5, 2021·Chemico-biological Interactions·Melania Iara FunkRomina María Uranga

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