Oxidation of an oligonucleotide-bound Ce(III)/multiphosphonate complex for site-selective DNA scission

Chemistry : a European Journal
Tuomas LönnbergMakoto Komiyama

Abstract

Oligodeoxyribonucleotide conjugates of ethylenediamine-N,N,N',N'-tetrakis(methylenephosphonic acid) (EDTP) have been used to place a Ce(III)/EDTP complex in close proximity to predetermined phosphodiester linkages of a complementary target oligonucleotide. In the presence of atmospheric oxygen, the Ce(III) is oxidized into Ce(IV) which, in turn, efficiently cleaves the target phosphodiester linkage. No cleavage occurs at the other single-stranded regions, which suggests that the catalytic Ce species is strictly localized next to the target phosphodiester linkage. No decrease in the reaction rate is observed upon introduction of scavengers for hydroxyl radicals (such as DMSO or MeOH) or singlet oxygen (such as NaN(3)) to the system; this indicates that the reaction proceeds via a hydrolytic pathway. Any significant contribution by an oxidative pathway is further ruled out by the observation that nucleosides remain intact after incubation with Ce(IV)/EDTP complex for extended periods.

References

Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D PeiP G Schultz
Sep 16, 2000·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·J X MengD X Feng
Apr 3, 2001·Current Opinion in Chemical Biology·S J Franklin
May 25, 2001·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·A Sreedhara, J A Cowan
Jul 16, 2003·Accounts of Chemical Research·Junghun Suh
Apr 6, 2004·Current Opinion in Chemical Biology·Janet R Morrow, Olga Iranzo
May 19, 2005·Chemical Communications : Chem Comm·Fabrizio MancinUmberto Tonellato
Jun 9, 2005·Journal of the American Chemical Society·Yan Jin, J A Cowan
Jul 21, 2005·Organic & Biomolecular Chemistry·Ana L Maldonado, Anatoly K Yatsimirsky
Aug 6, 2005·Nature Biotechnology·Matthew H Porteus, Dana Carroll
Apr 25, 2006·Nucleosides, Nucleotides & Nucleic Acids·Carl Elovson Grey, Patrick Adlercreutz
Dec 17, 2008·Organic & Biomolecular Chemistry·Tuomas LönnbergMakoto Komiyama
Dec 18, 2008·Dalton Transactions : an International Journal of Inorganic Chemistry·Changlin Liu, Li Wang

❮ Previous
Next ❯

Citations

Jul 10, 2012·Artificial DNA, PNA & XNA·Yuichiro AibaMakoto Komiyama
May 14, 2011·Chemical Society Reviews·Yuichiro AibaMakoto Komiyama
Oct 26, 2011·Future Medicinal Chemistry·Béla Gyurcsik, Anikó Czene
May 1, 2012·Chemical Communications : Chem Comm·Fabrizio MancinPaolo Tecilla

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.