PMID: 9169097Apr 1, 1997Paper

Oxidation of bilirubin by brain mitochondrial membranes--dependence on cell type and postnatal age

Biochemical and Molecular Medicine
T W Hansen, J W Allen

Abstract

Bilirubin is oxidized by brain mitochondrial membranes at a rate which may contribute significantly to clearance of bilirubin from the brain. Neurons appear to be more sensitive to bilirubin toxicity than glial cells. Clinical experience has suggested that sensitivity to bilirubin neurotoxicity may be greater in the neonate than later in life. We hypothesized that the ability to oxidize bilirubin would be lower in mitochondrial membranes from a pure neuronal compared to a mixed glial/neuronal source, and lower in immature than more mature brains. Mitochondria of synaptosomal and nonsynaptosomal origin were obtained from young rat brains by differential centrifugation in sucrose gradients. Synaptosomes were lysed by hypoosmotic treatment, and mitochondria were ruptured by sonication. The change in optical density of a bilirubin solution at 440 nm was measured over time following addition of the membrane suspension. The rate of bilirubin oxidation was significantly higher in nonsynaptic than in synaptic mitochondrial membranes [99.1 +/- 42.3 (mean +/- SD) vs 69.9 +/- 30.9 pmol/ min/mg protein, t = 4.835, P = 0.0003]. "Crude" mitochondrial membranes were obtained by differential centrifugation in sucrose from the forebrains of rat...Continue Reading

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Citations

Dec 6, 2000·European Journal of Biochemistry·K R KrancJ F Clark
Jun 11, 2010·BMC Research Notes·Safarina G MalikClaudio Tiribelli
Jan 5, 2002·Pediatric Research·Cecília M P RodriguesDora Brites
Mar 29, 2002·Pediatric Research·Adam Jonas, Delbert A Fisher
Nov 6, 2014·Archives of Toxicology·Sabrina E GambaroSilvia Gazzin
May 1, 2007·Neurobiology of Disease·Luna Benvenisti-Zarom, Raymond F Regan
Dec 7, 2006·Clinics in Perinatology·Jon F Watchko
Jan 9, 2003·Clinics in Perinatology·Thor Willy Ruud Hansen
Nov 24, 1999·Molecular Genetics and Metabolism·T W HansenS Tommarello
Sep 26, 2000·Molecular Genetics and Metabolism·T W Hansen
Feb 12, 1998·Biochemical and Molecular Medicine·T W HansenJ W Allen
Sep 1, 2016·Archives of Toxicology·Mohammed QaisiyaCristina Bellarosa
Jul 20, 2002·Molecular Pharmacology·John M SeubertJohn R Bend
Nov 5, 2019·Frontiers in Pharmacology·Xinyi LiShankai Yin

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