PMID: 9443077Jan 27, 1998Paper

Oxidation of glutamine in HeLa cells: role and control of truncated TCA cycles in tumour mitochondria

Journal of Cellular Biochemistry
T J Piva, E McEvoy-Bowe

Abstract

The oxidative metabolism of glutamine in HeLa cells was investigated using intact cells and isolated mitochondria. The concentrations of the cytoplasmic amino acids were found to be aspartate, 8.0 mM; glutamate, 22.2 mM; glutamine, 11.3 mM; glycine, 9.8 mM; taurine, 2.3 mM; and alanine, < 1 mM. Incubation of the cells with [14C]glutamine gave steady-state recoveries of 14C-label (estimated as exogenous glutamine) in the glutamine, glutamate, and aspartate pools, of 103%, 80%, and 25%, respectively, indicating that glutamine synthetase activity was absent and that a significant proportion of glutamate oxidation proceeded through aspartate aminotransferase. No label was detected in the alanine pool, suggesting that alanine aminotransferase activity was low in these cells. The clearance rate of [14C]glutamine through the cellular compartment was 65 nmol/min per mg protein. There was a 28 s delay after [14C]glutamine was added to the cell before 14C-label was incorporated into the cytoplasm, while the formation of glutamate commenced 10 s later. Aspartate was the major metabolite formed when the mitochondria were incubated in a medium containing either glutamine, glutamate, or glutamate plus malate. The transaminase inhibitor AOA i...Continue Reading

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Citations

May 24, 2005·Seminars in Cancer Biology·Sybille MazurekErich Eigenbrodt
Mar 22, 2002·The International Journal of Biochemistry & Cell Biology·José M MatésJavier Márquez
Feb 17, 2010·The International Journal of Biochemistry & Cell Biology·Sybille Mazurek
Jan 6, 2009·Molecular Nutrition & Food Research·Stephen J Ralph, Jiri Neuzil
Aug 4, 2004·Experimental Gerontology·Kevork HagopianRichard Weindruch
Jun 20, 2006·Free Radical Biology & Medicine·Nadezhda I FedotchevaMariya N Kondrashova
Feb 12, 2014·The International Journal of Biochemistry & Cell Biology·Rafael Moreno-SánchezSara Rodríguez-Enríquez
Sep 21, 2010·Chemistry & Biology·Mitsuhiro KitagawaMasaya Imoto
Oct 3, 2020·BMC Pharmacology & Toxicology·Haozhen ShaoHongxuan Tong

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