PMID: 6165705Jan 1, 1981Paper

Oxidation of ruthenium red for use as an intercellular tracer

Histochemistry
D A Handley, S Chien

Abstract

When ruthenium red (RR) is combined with OsO4, an electron-opaque complex forms which readily binds to the cell surface coat. However, the RR-OsO4 complex is often excluded from intercellular spaces in many cell types, and thus is not dependable as a tracer of regions continuous with the extracellular space. Postfixation of erythrocytes agglutinated by the lectin helix (Helix promatia) and intact carotid artery endothelium with a freshly prepared mixture of 1% OsO4 containing 0.1% ruthenium red (RR) resulted in a dense surface deposit of these cells, but intercellular regions were penetrated to a minimal degree by the stain. When a similar mixture of RR-OsO4 was allowed to stand 3 h before use, RR is oxidized by OsO4 to yield a ruthenium compound that has a spectrophotometric absorbance maximum at 365 nm. This RR molecule has a reduced number of cationic sites due to binding with osmium dioxide OsO2=. Postfixation of agglutinated RBCs and carotid artery endothelium with this oxidized ruthenium-OsO4 mixture resulted in a 50-80% decrease in surface deposition but markedly enhanced penetration into intercellular regions. The enhanced penetration is attributed to decreased binding affinity of the oxidized ruthenium for anionic surf...Continue Reading

References

Jun 9, 1976·The Journal of Membrane Biology·M Henkart, S Hagiwara
Jan 1, 1976·International Review of Cytology·J H Luft
Feb 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A J Nielson, W P Griffith
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Jan 1, 1979·Ultramicroscopy·D A Handley, B R Olsen
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Jan 1, 1971·The Journal of Cell Biology·F M MorelH Wayland

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Citations

Sep 1, 1989·Neuroscience and Behavioral Physiology·L A Fedosikhina, V A Otellin
May 1, 1986·Cell Differentiation·D MasquelierC Remacle
Jan 1, 1986·Acta histochemica·S DolapchievaK Ichev
Apr 1, 1988·American Journal of Hematology·W H Reinhart, S Chien
Nov 1, 1987·Metabolism: Clinical and Experimental·C M ArbeenyH A Eder

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