Jun 7, 1976

Oxidation of sarcosine and N-alkyl derivatives of glycine by D-amino-acid oxidase

Biochimica Et Biophysica Acta
M Naoi, K Yagi

Abstract

1. Sarcosine was oxidized by D-amino-acid oxidase (D-amino-acid: O2 oxidoreductase (deaminating), EC 1.4.3.3) to yield methylamine and glyoxylic acid. A seriies of N-alkyl glycines were also oxidized by this enzyme. 2. N-Acetyl- and N-Phenylglycine inhibited the oxidase by competing with the substrate, while N-methyl-N-acetylglycine did not bind to the enzyme. This suggests the requirement of at least one unsubstituted hydrogen atom at the amino group ofglycine for binding. 3. The primary step in the reaction was the release of a proton from the substrate, indicating the formation of a substituted imino acid, which was spontaneously hydrolyzed to glyoxylic acid acid and an amine.

  • References2
  • Citations6

Citations

Mentioned in this Paper

D-Amino Acid Dehydrogenase
Glycine, Monopotasssium Salt
Amines
Derivatives
Methylamines
DAO gene
Oxidase
Methylamine
Glycine
Oxidation

About this Paper

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