PMID: 8601759Jan 1, 1996

Oxidation reactions of a bovine serum albumin-bilirubin complex. A pulse radiolysis study

International Journal of Radiation Biology
S Adhikari, C Gopinathan

Abstract

Using the technique of pulse radiolysis, oxidation studies of the bovine serum albumin-bilirubin (BSA-BR) system with radicals like CCl3OO., N3., (SCN)2.-, Br2.- and OH. generated in neutral and alkaline medium are reported. In a neutral solution, BSA protects the bound BR very efficiently from the attack of these radicals. The experimental k/k' values for the reaction of CCl3OO., N3. and Br2.- radicals are 2.46, 1.78 and 2.55 respectively, where k and k' are the bimolecular rate constants for the formation of the semi-oxidized BSA and BR radicals respectively. The calculated ratios from our measurements of rate constants k and k' are 0.16, 0.28 and 1.38 for CCl3OO., N3. and Br2.- respectively. These values indicate protection of BR by BSA from free radical attack. For Br2.- radical-induced oxidation of the BSA-BR system, a radical transfer from protein to BR was observed. OH. shows very fast adduct formation with both BSA and BR. The bimolecular rate constant for the formation of BR-OH adducts at PH 8+/- 0.2 is 9.5 x 10(9) dm3 mol-1 s-1 (540 nm). OH. adds to BSA at neutral pH with a rate constant of 3.0 +/- 1.0 x 10(10) dm3 mol-1 s-1 (305 nm). In the BSA-BR complex, BSA fully protects BR from OH. attack and the (BSA-BR)-OH add...Continue Reading

References

Jul 1, 1979·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·E J Land, W A Prütz
Aug 1, 1977·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·E J Land, W A Prütz
Nov 1, 1976·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·C V EadsforthE W Thomas
Oct 1, 1989·International Journal of Radiation Biology·H Schuessler, E Jung
Nov 1, 1988·International Journal of Radiation Biology·M LalK D Asmus
Aug 1, 1987·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·H Schuessler, H Hartmann
Mar 1, 1983·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·H Schuessler, J V Davies
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B I GreeneC V Shank
Sep 16, 1980·Biochemical and Biophysical Research Communications·W A PrützA J Swallow

Related Concepts

Thiocyanogen ((SCN)2(1-))
Liver growth factor
Albumin Human, USP
Metazoa
Bilirubin, (4E,15E)-Isomer
Bromine
Bos indicus
Free Radicals
Growth Substances
Nitrogen

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.