Oxidation-reduction properties of several low potential iron-sulfur proteins and of methylviologen

N A StombaughW H Orme-Johnson


Apparent oxidation-reduction potentials at pH 7.0 and 25 degrees C were determined using the H2-hydrogenase system with ferredoxins from the following sources: Clostridium pasteurianum, -403 mV; C tartarovorum, -424 mV; C. acidi-urici, -434 mV; Peptococcus aerogenes, -427 mV; Chromatium D, -482 mV (pH 8.0); B. polymyxa, Fd I, -377 mV, and Fd II, -422 mV; and spinach, -428 mV. The pH dependence of these values was variable, ranging from -2 to -24 mV/pH unit increase for different ferredoxins. Over the range of buffer concentrations between 0.05 and 0.2 M, the potentials did not vary significantly. The number of electrons transferred during reduction (as determined by integrations of EPR spectra and by dithionite titration) is 2 for the first five proteins, while potentiometric data for all the cases fit a Nernst equation for which n = 1. The E degrees' value for the redox indicator methylviologen at pH 7.4 was found to be -460 mV, according to both the H2-hydrogenase system and cyclic voltammetry, significantly different from the value previously reported at higher pH's. Additionally, the presence of C. pasteuranum ferredoxin appears to shift the E degrees value of methylviologen to even more negative values. An analysis of sour...Continue Reading


May 17, 2008·Photosynthesis Research·Vasily V PtushenkoAlexey Yu Semenov
Jun 29, 2010·Journal of the American Chemical Society·Johannes W KungMatthias Boll
Sep 17, 2010·Nature Reviews. Microbiology·Korneel Rabaey, René A Rozendal
Aug 1, 1977·European Journal of Biochemistry·G ScheringsC Veeger
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·B R GibneyP L Dutton
May 22, 2013·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Bradley Scott Perrin, Toshiko Ichiye
Mar 21, 2007·Journal of Mass Spectrometry : JMS·Alfredo J IbáñezAles Svatos
Jul 17, 2010·Proteins·Bradley Scott Perrin, Toshiko Ichiye
Feb 1, 1979·Analytical Biochemistry·J S Chen, D K Blanchard
Apr 15, 2005·Biophysical Chemistry·Robert A Alberty
May 15, 1991·Archives of Biochemistry and Biophysics·C P Pace, M T Stankovich
May 7, 1985·Biochemistry·C T PrzysieckiM A Cusanovich
Jan 7, 2020·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Eric S BoydAlexis S Templeton
Aug 1, 1985·Quarterly Reviews of Biophysics·F A ArmstrongN J Walton


Aug 7, 1969·Biochemical and Biophysical Research Communications·W H Orme-Johnson, H Beinert
Jun 1, 1973·Analytical Chemistry·F M Hawkridge, T Kuwana
Jun 8, 1966·Biochimica Et Biophysica Acta·R Bachofen, D I Arnon
Dec 1, 1968·The Biochemical Journal·M C EvansF R Whatley
Jan 13, 1970·Biochimica Et Biophysica Acta·M T Borchert, J S Wessels
Jan 1, 1973·Annual Review of Biochemistry·W H Orme-Johnson
Jun 23, 1972·Biochimica Et Biophysica Acta·M Y TsoR H Burris
Apr 2, 1968·Biochimica Et Biophysica Acta·K Tagawa, D I Arnon
Jul 20, 1933·The Journal of General Physiology·L Michaelis, E S Hill

Related Concepts

Plasma Protein Binding Capacity
Protein Conformation
Electron Spin Resonance Spectroscopy
Pyridinium Compounds
Ferredoxin II

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.