PMID: 11342171May 9, 2001Paper

Oxidative and drug-induced alterations in brush border membrane hemileaflet fluidity, functional consequences for glucose transport

Biochimica Et Biophysica Acta
D Jourd'heuil, J B Meddings

Abstract

Oxidation of biological membranes has been suggested as a major pathological process in a variety of disease states including intestinal ischemia and inflammatory bowel disease. Previous studies on the small intestinal brush border membrane have shown that part of the decrease in the activity of the Na(+)-dependent glucose transporter (SGLT1) observed after oxidation could be secondary to the derangement in membrane fluidity that accompanied oxidative damage. The present study examined the relationship between oxidative-induced hemileaflet fluidity alterations and the resultant change in Na(+)-dependent glucose transport activity. To address this issue, in vitro oxidation of guinea pig brush border membrane vesicles was induced by incubation of the vesicles with ferrous sulfate and ascorbate. We found that oxidation decreased the fluidity of both the outer and inner hemileaflets, the decrease being greater in the outer leaflet. Moreover, the preferential alteration in hemileaflet fluidity was accompanied by a decrease in glucose transport. However, when membrane perturbing agents such as hexanol and A(2)C were used to restore membrane fluidity to levels comparable to controls, rates of glucose transport could not be interpreted...Continue Reading

References

Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·F Jähnig
Nov 16, 1979·Biochimica Et Biophysica Acta·R N Fontaine, F Schroeder
Jan 1, 1978·Methods in Enzymology·J A Buege, S D Aust
Oct 1, 1992·Kidney International·C Le GrimellecM C Giocondi
Jan 21, 1992·Biochemistry·E H el Yandouzi, C Le Grimellec
Apr 1, 1990·The Journal of Clinical Investigation·J B MeddingsS Thiesen
Oct 16, 1989·Biochemical and Biophysical Research Communications·M L WrattenA Sevanian
Mar 1, 1988·Digestive Diseases and Sciences·M B Grisham, D N Granger
Dec 8, 1988·Biochimica Et Biophysica Acta·F SchroederW G Wood
Jul 1, 1987·Chemistry and Physics of Lipids·C Richter
Nov 6, 1986·Biochimica Et Biophysica Acta·L I BarsukovG Semenza
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M P Sheetz, S J Singer
May 1, 1993·The American Journal of Clinical Nutrition·H Esterbauer
Jan 1, 1964·Analytical Biochemistry·A DAHLQVIST

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Citations

May 13, 2005·Archives of Toxicology·J BasivireddyK A Balasubramanian
Sep 19, 2012·Journal of Bioenergetics and Biomembranes·F A García-GilJ J García
Jul 27, 2005·Current Gastroenterology Reports·Gerald W DrydenCraig J McClain
Feb 27, 2004·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·A L KatynskiT W Moon
Jun 11, 2004·Annual Review of Nutrition·Elizabeth C Theil
Jan 27, 2010·Biochimica Et Biophysica Acta·Guanghua Zhao
Nov 19, 2003·AAPS PharmSci·Vanessa M D'SouzaGiovanni M Pauletti
Feb 28, 2004·Experimental Biology and Medicine·Yasuhiro KimuraRandal K Buddington

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