PMID: 7539421Jun 1, 1995Paper

Oxidative cell wall damage mediated by bleomycin-Fe(II) in Saccharomyces cerevisiae

Journal of Bacteriology
S T LimP N Lipke

Abstract

Bleomycin mediates cell wall damage in the yeast Saccharomyces cerevisiae. Bleomycin treatments in the presence of Fe(II) increased the rate of spheroplast formation by lytic enzymes by 5- to 40-fold. Neither Fe(III) nor other tested ions caused significant cell wall damage in the presence of bleomycin. The effect of bleomycin-Fe(II) on the cell wall mimicked the characteristics of bleomycin-Fe(II)-mediated DNA damage in dependence on aeration, inhibition by ascorbate, and potentiation by submillimolar concentrations of sodium phosphate. Bleomycin-mediated cell wall damage was time and dose dependent, with incubations as short as 20 min and drug concentrations as low as 3.3 x 10(-7)M causing measurable cell wall damage in strain CM1069-40. These times and concentrations are within the range of effectiveness for bleomycin-mediated DNA damage and for the cytotoxicity of the drug. Although Fe(III) was inactive with bleomycin and O2, the bleomycin-Fe(III) complex damaged walls and lysed cells in the presence of H2O2. H2O2 causes similar activation of bleomycin-Fe(III) in assays of DNA scission. These results suggest that an activated bleomycin-Fe-O2 complex disrupts essential cell wall polymers in a manner analogous to bleomycin-me...Continue Reading

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Citations

Jun 13, 2002·Folia Microbiologica·E StavrinidisE Tiligada
Jun 20, 1998·Molecular and Cellular Biology·W Zheng, S A Johnston
Sep 25, 2003·Antimicrobial Agents and Chemotherapy·Carol W MooreRonald E Gordon
Mar 16, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Teodora TodorovaStephka Chankova
Jul 31, 1999·Applied and Environmental Microbiology·R OvalleP N Lipke
May 11, 1999·Drug Discovery Today·J A Heinemann
Feb 19, 2002·Chemical Reviews·Richard M. Burger

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