PMID: 8596543Jan 1, 1996Paper

Oxidative damage to DNA induced by areca nut extract

Mutation Research
T Y LiuC W Chi

Abstract

In Taiwan, people chew betel quid which contains tender areca nut with husk. In other countries, people prefer ripe and dried areca nut without husk. In this study, we compared the reactive oxygen species-induced oxidative DNA damage in isolated DNA and CHO-K1 cells between treatments with tender areca nut extract (ANE) and ripe ANE. Incubation of these two ANE preparations with isolated DNA generated 8-hydroxy-2'-deoxyguanosine (8-OH-dG) in an alkaline environment in a dose-dependent manner. Ripe ANE generated higher levels of 8-OH-dG compared to tender ANE. The addition of iron(II) (100 microM) resulted in 1.4- and 3.1-fold increases of 8-OH-dG when incubated with 1 mg/ml each of tender and ripe ANE. In testing the effect of ANE to cellular DNA, CHO-K1 cells were used for its documented sensitivity to reactive oxygen species. In CHO-K1 cells, ripe ANE was more cytotoxic than tender ANE following an 18-h incubation. The cytotoxicity to CHO-K1 cells was positively correlated with the formation of 8-OH-dG following tender (r=0.97) and ripe (r=0.91) ANE treatment. Addition of the iron chelating agent o-phenanthroline (10 and 20 microM) to cells prior to ri ANE exposure significantly increased (p<0.05) the survival of CHO-K1 cells...Continue Reading

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Citations

Oct 16, 2013·Oral Oncology·Sheetal Korde ChoudhariSatyajit Tekade
Sep 25, 1999·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·T Y LiuC W Chi
Dec 12, 2001·Oral Microbiology and Immunology·L J LingY L Lai
Mar 26, 2002·Journal of Toxicology and Environmental Health. Part a·Chiu-Lan ChenTsung-Yun Liu
Jul 15, 2006·Journal of Toxicology and Environmental Health. Part a·Ping-Ho ChenChung-Ho Chen
Jun 27, 2013·Molecular Cancer·I-Chieh ChenHung-Che Chiang
Apr 6, 2006·Journal of Periodontology·Shan-Ling HungYen-Ting Chen
Aug 13, 2014·TheScientificWorldJournal·Ping-Ho ChenBin Huang
Dec 4, 2010·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Yu-Chi LiuYoung-Chau Liu
Mar 9, 2010·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Kuang-Ping PengTsan-Zon Liu
Oct 21, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Pei-Ling HuangTsung-Yun Liu
Aug 6, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Chia-Chi WangTong-Rong Jan
May 5, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Chia-Chi WangTong-Rong Jan
Apr 13, 2011·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Chia-Chi WangTong-Rong Jan
Feb 18, 2014·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Wan-Chun LiKuo-Wei Chang
May 6, 2004·Annals of the New York Academy of Sciences·Duan-Jun TanLee-Jun C Wong
Dec 5, 2013·International Journal of Cancer. Journal International Du Cancer·Neela GuhaKurt Straif
Aug 14, 2013·Journal of Immunotoxicology·Chin-Cheng LeeChia-Chi Wang
May 16, 2002·Toxicology and Applied Pharmacology·James A Mobley, Robert W Brueggemeier

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