Oxidative damages in chronic inflammation of a mouse autoimmune disease model

Immunology Letters
Minyi ShiToru Takeuchi

Abstract

Reactive oxygen species are generated in many types of inflammation; it is unclear, however, if inflammation leads to oxidative damage of DNA, proteins and lipids within the inflamed tissues. In this study, we used mice that are homozygous for the alymphoplasia (aly) mutation as a model to determine if inflammation induces oxidative damage in liver and pancreas. We found that 8-hydroxy-2'-deoxyguanosine (8OHdG), which is a product of oxidative DNA damage, increases with age in livers and pancreata of C57BL/6aly/aly (aly/aly) and C57BL/6 wild type (WT) mice. The 8OHdG levels in liver, but not in pancreas, of aged aly/aly mice were significantly higher than those in age-matched WT mice. We showed that aging enhances oxidative protein damage, as measured by carbonylated protein contents, in the pancreata of WT but not aly/aly mice. In contrast, neither aging nor inflammation was associated with lipid damage, as measured by thiobarbituric acid-reactive substances (TBARS), in aly/aly or WT mice. Our results indicate that chronic inflammation in liver but not pancreas leads to increased oxidative damage to DNA, but not to lipids and proteins in aly/aly mouse model.

References

Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C G FragaB N Ames
Sep 1, 1993·Annals of the Rheumatic Diseases·S BashirP G Winyard
Feb 17, 1996·Lancet·P DandonaT Nicotera
May 13, 1998·Mechanisms of Ageing and Development·T Hayashi, T Miyazawa
May 11, 1999·The Journal of Laboratory and Clinical Medicine·O NiemeläB Bacon
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·M L HamiltonA Richardson
Jul 2, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marcus S CookeJoseph Lunec

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Citations

Jul 17, 2007·Cells, Tissues, Organs·Yoichi NakamuraMasahiro Itoh
Sep 1, 2007·Environmental Health and Preventive Medicine·Minyi ShiToru Takeuchi
Aug 15, 2009·Free Radical Research·Kimiko IzumoToru Takeuchi

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