Abstract
Reactive oxygen species are generated in many types of inflammation; it is unclear, however, if inflammation leads to oxidative damage of DNA, proteins and lipids within the inflamed tissues. In this study, we used mice that are homozygous for the alymphoplasia (aly) mutation as a model to determine if inflammation induces oxidative damage in liver and pancreas. We found that 8-hydroxy-2'-deoxyguanosine (8OHdG), which is a product of oxidative DNA damage, increases with age in livers and pancreata of C57BL/6aly/aly (aly/aly) and C57BL/6 wild type (WT) mice. The 8OHdG levels in liver, but not in pancreas, of aged aly/aly mice were significantly higher than those in age-matched WT mice. We showed that aging enhances oxidative protein damage, as measured by carbonylated protein contents, in the pancreata of WT but not aly/aly mice. In contrast, neither aging nor inflammation was associated with lipid damage, as measured by thiobarbituric acid-reactive substances (TBARS), in aly/aly or WT mice. Our results indicate that chronic inflammation in liver but not pancreas leads to increased oxidative damage to DNA, but not to lipids and proteins in aly/aly mouse model.
References
Jul 1, 1992·The Journal of Experimental Medicine·J D MacMickingW B Cowden
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C G FragaB N Ames
Sep 1, 1993·Annals of the Rheumatic Diseases·S BashirP G Winyard
Feb 17, 1996·Lancet·P DandonaT Nicotera
Apr 1, 1996·Carcinogenesis·M NakajimaK Morimoto
Nov 1, 1996·European Journal of Immunology·R TsubataT Honjo
May 13, 1998·Mechanisms of Ageing and Development·T Hayashi, T Miyazawa
May 11, 1999·The Journal of Laboratory and Clinical Medicine·O NiemeläB Bacon
May 13, 1999·Nature Genetics·R ShinkuraT Honjo
Aug 22, 2000·Acta Physiologica Scandinavica·J BejmaL L Ji
Jul 7, 2001·Free Radical Biology & Medicine·S M DaviesM P Murphy
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·M L HamiltonA Richardson
Nov 3, 2001·Journal of Hepatology·T KitadaK Wakasa
Jan 10, 2002·The Journal of Experimental Medicine·Satish DevadasMark S Williams
Mar 26, 2003·Histopathology·S SekiK Wakasa
Jul 2, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marcus S CookeJoseph Lunec
Citations
Jul 17, 2007·Cells, Tissues, Organs·Yoichi NakamuraMasahiro Itoh
Sep 1, 2007·Environmental Health and Preventive Medicine·Minyi ShiToru Takeuchi
Jan 27, 2016·Pharmaceutical Biology·Yan FengYi Liu
Aug 15, 2009·Free Radical Research·Kimiko IzumoToru Takeuchi