Oxidative DNA damage correlates with cell immortalization and mir-92 expression in hepatocellular carcinoma.

BMC Cancer
R CardinF Farinati

Abstract

MicroRNAs expression has been extensively studied in hepatocellular carcinoma but little is known regarding the relationship, if any, with inflammation, production of reactive oxygen species (ROS), host's repair mechanisms and cell immortalization. This study aimed at assessing the extent of oxidative DNA damage (8-hydroxydeoxyguanosine - 8-OHdG) in different phases of the carcinogenetic process, in relation to DNA repair gene polymorphism, telomeric dysfunction and to the expression of several microRNAs, non-coding genes involved in post-transcriptional regulation, cell proliferation, differentiation and death. Tissue samples obtained either at surgery, [neoplastic (HCC) and adjacent non-cancerous cirrhotic tissues (NCCT)] at percutaneous or laparoscopic biopsy (patients with HCV or HBV-related hepatitis or patients undergoing cholecystectomy) were analysed for 8-OHdG (HPLC-ED), OGG1 (a DNA repair gene) polymorphism (PCR-RFLP), telomerase activity, telomere length (T/S, by RT-PCR), Taqman microRNA assay and Bad/Bax mRNA (RT-PCR). Fifty-eight samples from 29 HCC patients (obtained in both neoplastic and peritumoral tissues), 22 from chronic hepatitis (CH) and 10 controls (cholecystectomy patients - CON) were examined. Eight-OHd...Continue Reading

References

Feb 16, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Y W LinF J Lu
Dec 18, 2001·Environmental and Molecular Mutagenesis·S MitraT K Hazra
May 10, 2002·Nucleic Acids Research·Richard M Cawthon
May 22, 2003·Cancer Letters·Kenkichi Masutomi, William C Hahn
Aug 15, 2003·World Journal of Gastroenterology : WJG·Dao-Yong LiuChong-Zhi Zhou
Apr 2, 2004·Biological & Pharmaceutical Bulletin·Hiroshi Ide, Mitsuharu Kotera
Jun 9, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Christoph JüngstWolfgang Helmut Caselmann
Apr 13, 2006·World Journal of Gastroenterology : WJG·Fabio FarinatiMassimo Rugge
Jun 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lin ZhangGeorge Coukos
Aug 31, 2006·Molecular and Cellular Biology·Anwar HossainGrady F Saunders
Jan 11, 2007·Free Radical Biology & Medicine·Naoki FujitaMasahiko Kaito
Feb 9, 2007·Journal of Gastroenterology·Yutaka Sasaki
Dec 12, 2007·Journal of Viral Hepatitis·F FarinatiR Naccarato
Jan 8, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Anuradha BudhuXin Wei Wang
Jan 29, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jinmai JiangThomas D Schmittgen
Mar 12, 2008·Journal of Viral Hepatitis·N FujitaY Takei
Mar 28, 2008·International Journal of Cancer. Journal International Du Cancer·Fabio FarinatiMassimo Rugge
Apr 15, 2008·Future Oncology·Imran A BabarJoanne B Weidhaas
Apr 25, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yannick LadeiroJessica Zucman-Rossi
May 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Eun Ji NamSunghoon Kim
May 29, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Chiara Braconi, Tushar Patel
Dec 17, 2008·Cancer Letters·Mehmet OzturkHaluk Yuzugullu
Jan 6, 2009·Journal of Cellular and Molecular Medicine·Laura GramantieriMassimo Negrini
Jan 15, 2009·Liver International : Official Journal of the International Association for the Study of the Liver·Taichiro NishikawaToshikazu Yoshikawa
Apr 21, 2009·International Journal of Cancer. Journal International Du Cancer·Takahiro IchimiNaohiko Seki
May 15, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Teng XuShi-Mei Zhuang
Jul 9, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Junfang JiXin Wei Wang
Jul 18, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Isabella ManniAda Sacchi
Jul 25, 2009·Liver International : Official Journal of the International Association for the Study of the Liver·Nitin SainiArvind Rajwanshi
Jul 31, 2009·Oncogene·N N Danial
Sep 29, 2009·Mechanisms of Ageing and Development·Guorong LiPedro Gonzalez
Nov 6, 2009·Carcinogenesis·Steven E Artandi, Ronald A DePinho
Dec 19, 2009·Toxicologic Pathology·James E KlaunigBarbara A Hocevar
Jan 9, 2010·Breast Cancer Research and Treatment·Weiguang YuanDianjun Li
Mar 17, 2010·Oncogene·W-L Tsai, R T Chung
Apr 3, 2010·BMC Gastroenterology·Andromachi KotsaftiMarina Bortolami
Apr 8, 2010·Free Radical Research·Geou-Yarh Liou, Peter Storz
Sep 3, 2010·Annual Review of Pharmacology and Toxicology·Michela Garofalo, Carlo M Croce
Oct 15, 2010·Expert Reviews in Molecular Medicine·Bo Song, Jingfang Ju

❮ Previous
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Citations

Mar 27, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Wei-Xian ChenJian-Hua Zhao
Jun 8, 2013·Annals of Surgical Oncology·Romilda CardinFabio Farinati
Aug 29, 2013·World Journal of Gastroenterology : WJG·Catherine M GreeneMatthew W Lawless
Apr 4, 2014·World Journal of Gastroenterology : WJG·Romilda CardinFabio Farinati
Sep 12, 2012·PPAR Research·Francesca AgriestiClaudia Piccoli
Aug 2, 2014·Pharmacogenomics·Celestino SarduGiuseppe Paolisso
Apr 14, 2015·Seminars in Cancer Biology·Stephanie C CaseyDean W Felsher
Jul 21, 2015·Liver International : Official Journal of the International Association for the Study of the Liver·Kendall R Van Keuren-JensenJorge Rakela
Sep 27, 2015·Translational Research : the Journal of Laboratory and Clinical Medicine·Marika PiciocchiFabio Farinati
Jan 23, 2016·Oxidative Medicine and Cellular Longevity·Marco TafaniMatteo A Russo
Jun 25, 2015·Carcinogenesis·Sabine A S LangieAndrew R Collins
Jun 13, 2015·Diagnostic Pathology·Hua YeXuebao Zheng
May 13, 2014·Free Radical Biology & Medicine·Jinah ChoiYiyan Wang
Jun 6, 2013·International Journal of Molecular Sciences·Joanna DanielssonOnni Niemelä
Oct 31, 2015·Surgery Today·Masayuki NagahashiToshifumi Wakai
Dec 15, 2016·Oncotarget·Alexander V IvanovMaria G Isaguliants
May 13, 2020·Journal of Cellular Physiology·Seyed Omar EbrahimiSalar Shareef
Aug 9, 2017·Oxidative Medicine and Cellular Longevity·Anna KakehashiHideki Wanibuchi
Dec 14, 2016·Oxidative Medicine and Cellular Longevity·Zhanpeng WangWei Li
May 15, 2018·International Journal of Cancer. Journal International Du Cancer·Xiao MaYong-Bing Xiang
Dec 7, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Liam CornellHelen L Reeves
Jul 19, 2019·Molecular Therapy. Nucleic Acids·Huaping LiDao Wen Wang
Sep 28, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Nabil M Abdel-HamidDaniah Muneam Hamid

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Methods Mentioned

BETA
surgical
biopsy
surgical resection
ELISA
PCR
genotyping
biopsies
electrophoresis
Protein Assay

Software Mentioned

ABI 7900 SDS

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