Oxidative DNA damage in osteoarthritic porcine articular cartilage.

Journal of Cellular Physiology
Antonia F ChenBeverley Fermor

Abstract

Osteoarthritis (OA) is associated with increased levels of reactive oxygen species. This study investigated if increased oxidative DNA damage accumulates in OA articular cartilage compared with non-OA articular cartilage from pigs with spontaneous OA. Additionally, the ability of nitric oxide (NO) or peroxynitrite (ONOO(-)) induced DNA damage in non-OA chondrocytes to undergo endogenous repair was investigated. Porcine femoral condyles were graded for the stage of OA, macroscopically by the Collins Scale, and histologically by the modified Mankin Grade. Levels of DNA damage were determined in non-OA and OA cartilage, using the comet assay. For calibration, DNA damage was measured by exposing non-OA chondrocytes to 0-12 Gray (Gy) of X-ray irradiation. Non-OA articular chondrocytes were treated with 0-500 microM of NO donors (NOC-18 or SIN-1), and DNA damage assessed after treatment and 5 days recovery. A significant increase (P < 0.01) in oxidative DNA damage occurred in OA chondrocytes in joints with Mankin Grades 3 or greater, compared to non-OA chondrocytes. The percentage of nuclei containing DNA damage increased significantly (P < 0.001) from early to late grades of OA. An increase of approximately 0.65-1.7 breaks/1,000 kb ...Continue Reading

References

Jan 1, 1992·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·J A van der SluijsJ Drukker
Aug 1, 1996·Methods : a Companion to Methods in Enzymology·C H Evans, M Stefanovic-Racic
Apr 29, 1997·Mutation Research·A R CollinsR Stĕtina
Jul 27, 1999·Osteoarthritis and Cartilage·M LotzK Kühn
Jul 27, 1999·Osteoarthritis and Cartilage·J PelletierJ Martel-Pelletier
Sep 26, 2000·American Journal of Physiology. Cell Physiology·R K StuderC H Evans
Oct 4, 2000·Neurobiology of Aging·K SchindowskiA Eckert
Aug 24, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·B FermorF Guilak
Nov 16, 2001·Current Rheumatology Reports·S B AbramsonR Clancy
Feb 13, 2002·Arthritis and Rheumatism·Marcello Del Carlo, Richard F Loeser
Sep 5, 2002·Arthritis and Rheumatism·T Aigner, H A Kim
Dec 31, 2002·Mutation Research·Krzysztof KońcaAndrzej Wojcik
Sep 18, 2003·Osteoarthritis and Cartilage·Y E HenrotinJ-P L Pujol
Dec 17, 2003·Clinical Rheumatology·Ayşe KaranAhmet Oncel
Apr 9, 2004·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·James A MartinJoseph A Buckwalter
Jul 3, 2004·Clinical Orthopaedics and Related Research·Farshid GuilakJ Brice Weinberg
Jan 11, 2005·Osteoarthritis and Cartilage·M Mathy-HartertY Henrotin
Jul 9, 2005·Nucleic Acids Research·Toru IshibashiMutsuo Sekiguchi
Dec 13, 2006·Medical Physics·Ming De LinG Allan Johnson
Jan 24, 2007·Physiological Reviews·Pál PacherLucas Liaudet
Apr 12, 2007·European Cells & Materials·B FermorJ B Weinberg
Jun 30, 2007·Nature Clinical Practice. Rheumatology·Thomas AignerJochen Haag
Jan 1, 2008·Arthritis and Rheumatism·Reva C LawrenceUNKNOWN National Arthritis Data Workgroup

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Citations

Mar 1, 2012·Histochemistry and Cell Biology·Stefan Hübner, Athina Efthymiadis
Aug 27, 2013·Arthritis Research & Therapy·Mandy PeffersPeter Clegg
Jan 16, 2016·Biochimica Et Biophysica Acta·Panagiotis Lepetsos, Athanasios G Papavassiliou
May 30, 2009·Free Radical Biology & Medicine·Lu Miao, Daret K St Clair
Jul 5, 2012·Journal of Tissue Engineering and Regenerative Medicine·Ronny M SchulzDaniel Huster
Jul 17, 2010·Osteoarthritis and Cartilage·B FermorB O Diekman
Mar 6, 2018·Expert Opinion on Therapeutic Targets·Andreas LeonidouEleftherios Tsiridis
Aug 18, 2018·Experimental and Therapeutic Medicine·Ying HeJinghong Chen
Dec 21, 2018·Molecular Medicine Reports·Jun Li, Jianping Zheng
Nov 24, 2020·Osteoarthritis and Cartilage·M E CoppB O Diekman

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